Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching

K. Jacobson, Z. Derzko, E. S. Wu, Y. Hou, George Poste

Research output: Chapter in Book/Report/Conference proceedingChapter

103 Scopus citations

Abstract

The use of fluorescence recovery after photobleaching (FRAP) techniques to monitor the lateral mobility of plant lectin receptor complexes on the surface of single, living mammalian cells is described in detail. FRAP measurements indicate that over 75% of the wheat germ agglutinin receptor (WGA receptor) complexes on the surface of human embryo fibroblasts are mobile. These WGA receptor complexes diffuse laterally (as opposed to flow) on the cell surface with a diffusion coefficient in the range of 2 x 10 -11 to 2 x 10 -10 cm 2/sec. Both the percentage of mobile WGA receptor complexes and the mean diffusion coefficient of these complexes are higher than that obtained from earlier FRAP measurements of the mobility of concanavalin A receptor (Con A receptor) complexes in a variety of cell types. The possible reasons for the differing mobilities of WGA and Con A receptors are discussed.

Original languageEnglish (US)
Title of host publicationJournal of Supramolecular and Cellular Biochemistry
Pages565-576
Number of pages12
Volume5
Edition4
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Jacobson, K., Derzko, Z., Wu, E. S., Hou, Y., & Poste, G. (1977). Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching. In Journal of Supramolecular and Cellular Biochemistry (4 ed., Vol. 5, pp. 565-576)