Measurement of gonadal hormones in dried blood spots versus serum: Verification of menstrual cycle phase

Elizabeth A. Shirtcliff, Rebecca Reavis, William H. Overman, Douglas A. Granger

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

In the present study, we show that blood spot assays for estradiol, progesterone, and testosterone are a reliable, accurate, and sensitive means for measuring circulating gonadal hormones. The lower limit of sensitivity of each blood spot assay is sufficient to determine gonadal hormone levels in adult females. Correspondence of serum to blood spot measures is high, with blood spot hormone levels explaining an average of 88.60% of the variance in serum gonadal hormones in females, but only 46.20% in males. We provide formulas for converting hormone levels in blood to hormone levels in serum (which traditional endocrinology studies report). Finally, we show that careful attempts to estimate hormone status by day-count methods are unreliable when compared to hormone assay in blood spots.

Original languageEnglish (US)
Pages (from-to)258-266
Number of pages9
JournalHormones and Behavior
Volume39
Issue number4
DOIs
StatePublished - 2001

Keywords

  • Dried blood spots
  • Estradiol
  • Menstrual cycle phase
  • Progesterone
  • Serum
  • Testosterone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

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