Measurement of ascorbic acid concentration and glutathione peroxidase activity in biological samples collected from horses with recurrent airway obstruction

Rachel H H Tan, Craig Thatcher, Virginia Buechner-Maxwell, Undine Christmann, Mark V. Crisman, Stephen R. Werre

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective - To measure the ascorbic acid (AA) concentration in bronchoalveolar lavage fluid (BALF) and cellular glutathione peroxidase (cGPx) activity in RBCs and WBCs from peripherally obtained blood and in cells from BALF to determine whether differences existed between the 2 major redox systems in recurrent airway obstruction (RAO)-affected and -nonaffected (control) horses and between systemic and local pulmonary responses in the glutathione redox system. Animals - 16 adult horses in pairs: 8 healthy (control) and 8 RAO-affected horses. Procedures - Physical examination data and biological samples were collected from horses before (remission), during, and after (recovery) environmental challenge with dusty straw and hay. At each stage, BALF cell AA concentration and RBC, WBC, and BALF cell cGPx activity were measured. Results - Compared with control horses, RAO-affected horses had significantly higher cGPx activity in RBCs at all points and in WBCs during remission and challenge. The BALF cell cGPx activity was higher in RAO-affected horses during recovery than during remission. The BALF cell AA concentration did not differ significantly in control horses at any point, but total and free AA concentrations were significantly lower in RAO-affected horses during the challenge period than during remission and recovery periods. Conclusions and Clinical Relevance - High cGPx activity suggested this redox system was upregulated during exposure to dusty straw and hay to combat oxidative stress, as AA was depleted in RAO-affected horses. The relative delay and lack of comparative increase in cGPx activity within the local environment (represented by BALF cells), compared with that in RBCs and WBCs, might contribute to disease in RAO-affected horses.

Original languageEnglish (US)
Pages (from-to)1500-1507
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume71
Issue number12
DOIs
StatePublished - Dec 2010

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Airway Obstruction
Glutathione Peroxidase
glutathione peroxidase
Ascorbic Acid
Horses
bioactive properties
ascorbic acid
Bronchoalveolar Lavage Fluid
horses
remission
sampling
leukocytes
erythrocytes
Oxidation-Reduction
cells
hay
straw
fluids
clinical examination
Physical Examination

ASJC Scopus subject areas

  • veterinary(all)

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Measurement of ascorbic acid concentration and glutathione peroxidase activity in biological samples collected from horses with recurrent airway obstruction. / Tan, Rachel H H; Thatcher, Craig; Buechner-Maxwell, Virginia; Christmann, Undine; Crisman, Mark V.; Werre, Stephen R.

In: American Journal of Veterinary Research, Vol. 71, No. 12, 12.2010, p. 1500-1507.

Research output: Contribution to journalArticle

Tan, Rachel H H ; Thatcher, Craig ; Buechner-Maxwell, Virginia ; Christmann, Undine ; Crisman, Mark V. ; Werre, Stephen R. / Measurement of ascorbic acid concentration and glutathione peroxidase activity in biological samples collected from horses with recurrent airway obstruction. In: American Journal of Veterinary Research. 2010 ; Vol. 71, No. 12. pp. 1500-1507.
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