Matrix metalloproteinase 2 secretion in WEHI 164 fibrosarcoma cells is nitric oxide-related and modified by morphine

Ahmad Shariftabrizi, Artemissia Phoebe Nifli, Mohammad Ansari, Farshid Saadat, Mohammad Reza Ebrahimkhani, Nastaran Alizadeh, Azadeh Nasseh, Vassilia Ismini Alexaki, Ahmad Reza Dehpour, Elias Castanas, Mohammad Reza Khorramizadeh

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Matrix metalloproteinases (MMP) are ubiquitous enzymes involved in extracellular matrix remodeling, and as a consequence in a number of physiological and pathological states, including development, wound healing and cancer. A crucial feature of cancer progression and metastasis is the disruption of extracellular matrix, and spreading of proliferating cancer cells. Modulation of MMP is a main target of cancer research. Using the mouse fibrosarcoma cell line WEHI 164, producing high amounts of MMP-2, we investigated whether we could modulate its production. We report that MMP-2 is under the control of nitric oxide (NO)/nitric oxide synthase (NOS) system. In addition, we show that NOS activity is controlled by opioids in a non-opioid receptor-related manner. Finally, we provide evidence that morphine, when administrated at low, non-toxic concentrations (< 10- 9 M) attenuates MMP-2 activity. We conclude that, as morphine is able to decrease metalloproteinase activity via the NO/NOS system, it may have a place in the treatment of several sarcomas including fibrosarcoma.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalEuropean Journal of Pharmacology
Volume530
Issue number1-2
DOIs
StatePublished - Jan 13 2006

Keywords

  • Fibrosarcoma cell (WEFI 164)
  • Nitric oxide
  • Nitric oxide synthase
  • Opioid (morphine)
  • Opioid receptors (mu, delta, kappa)

ASJC Scopus subject areas

  • Pharmacology

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  • Cite this

    Shariftabrizi, A., Nifli, A. P., Ansari, M., Saadat, F., Reza Ebrahimkhani, M., Alizadeh, N., Nasseh, A., Alexaki, V. I., Dehpour, A. R., Castanas, E., & Khorramizadeh, M. R. (2006). Matrix metalloproteinase 2 secretion in WEHI 164 fibrosarcoma cells is nitric oxide-related and modified by morphine. European Journal of Pharmacology, 530(1-2), 33-39. https://doi.org/10.1016/j.ejphar.2005.11.043