Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein

E. Watson, B. Shah, R. DePrince, R. W. Hendren, R. Nelson

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31 Scopus citations

Abstract

Matrix-assisted laser desorption ionization mass spectrometry (MALDI) has been investigated as a technique for the characterization of recombinant stem cell factor that had been covalently modified with polyethylene glycol (PEG) chains. The attachment of PEG chains produces a heterogeneous mixture of protein species that differ by 6000 Da. These differences in molecular weight could be readily determined by MALDI. The potential of MALDI as a general strategy for characterizing PEG-modified proteins is discussed.

Original languageEnglish (US)
Pages (from-to)278-281
Number of pages4
JournalBioTechniques
Volume16
Issue number2
StatePublished - Jan 1 1994

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Watson, E., Shah, B., DePrince, R., Hendren, R. W., & Nelson, R. (1994). Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein. BioTechniques, 16(2), 278-281.