Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein

E. Watson, B. Shah, R. DePrince, R. W. Hendren, R. Nelson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Matrix-assisted laser desorption ionization mass spectrometry (MALDI) has been investigated as a technique for the characterization of recombinant stem cell factor that had been covalently modified with polyethylene glycol (PEG) chains. The attachment of PEG chains produces a heterogeneous mixture of protein species that differ by 6000 Da. These differences in molecular weight could be readily determined by MALDI. The potential of MALDI as a general strategy for characterizing PEG-modified proteins is discussed.

Original languageEnglish (US)
Pages (from-to)278-281
Number of pages4
JournalBioTechniques
Volume16
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Recombinant Proteins
Ionization
Mass spectrometry
Desorption
Lasers
Stem Cell Factor
Proteins
Molecular Weight
Molecular weight

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

Watson, E., Shah, B., DePrince, R., Hendren, R. W., & Nelson, R. (1994). Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein. BioTechniques, 16(2), 278-281.

Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein. / Watson, E.; Shah, B.; DePrince, R.; Hendren, R. W.; Nelson, R.

In: BioTechniques, Vol. 16, No. 2, 1994, p. 278-281.

Research output: Contribution to journalArticle

Watson, E, Shah, B, DePrince, R, Hendren, RW & Nelson, R 1994, 'Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein', BioTechniques, vol. 16, no. 2, pp. 278-281.
Watson E, Shah B, DePrince R, Hendren RW, Nelson R. Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein. BioTechniques. 1994;16(2):278-281.
Watson, E. ; Shah, B. ; DePrince, R. ; Hendren, R. W. ; Nelson, R. / Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein. In: BioTechniques. 1994 ; Vol. 16, No. 2. pp. 278-281.
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