Mass Spectra and Yields of Intact Charged Biomolecules Ejected by Massive Cluster Impact for Bioimaging in a Time-of-Flight Secondary Ion Microscope

Jitao Zhang, Klaus Franzreb, Sergei A. Aksyonov, Peter Williams

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Impacts of massive, highly charged glycerol clusters (≳106 Da, ≳ ± 100 charges) have been used to eject intact charged molecules of peptides, lipids, and small proteins from pure solid samples, enabling imaging using these ion species in a time-of-flight secondary ion microscope with few-micrometer spatial resolution. Here, we report mass spectra and useful ion yields (ratio of intact charged molecules detected to molecules sputtered) for several molecular species-two peptides, bradykinin and angiotensin II; two lipids, phosphatidylcholine and sphingomyelin; Irganox 1010 (a detergent); insulin; and rhodamine B-and show that useful ion yields are high enough to enable bioimaging of peptides and lipids in biological samples with few-micrometer resolution and acceptable signals. For example, several hundred molecular ion counts should be detectable from a 3 × 3 μm2 area of a pure lipid bilayer given appropriate instrumentation or tens of counts from a minor constituent of such a layer.

Original languageEnglish (US)
Pages (from-to)10779-10784
Number of pages6
JournalAnalytical Chemistry
Volume87
Issue number21
DOIs
StatePublished - Oct 9 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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