TY - JOUR
T1 - Mass Spectra and Yields of Intact Charged Biomolecules Ejected by Massive Cluster Impact for Bioimaging in a Time-of-Flight Secondary Ion Microscope
AU - Zhang, Jitao
AU - Franzreb, Klaus
AU - Aksyonov, Sergei A.
AU - Williams, Peter
PY - 2015/10/9
Y1 - 2015/10/9
N2 - Impacts of massive, highly charged glycerol clusters (≳106 Da, ≳ ± 100 charges) have been used to eject intact charged molecules of peptides, lipids, and small proteins from pure solid samples, enabling imaging using these ion species in a time-of-flight secondary ion microscope with few-micrometer spatial resolution. Here, we report mass spectra and useful ion yields (ratio of intact charged molecules detected to molecules sputtered) for several molecular species-two peptides, bradykinin and angiotensin II; two lipids, phosphatidylcholine and sphingomyelin; Irganox 1010 (a detergent); insulin; and rhodamine B-and show that useful ion yields are high enough to enable bioimaging of peptides and lipids in biological samples with few-micrometer resolution and acceptable signals. For example, several hundred molecular ion counts should be detectable from a 3 × 3 μm2 area of a pure lipid bilayer given appropriate instrumentation or tens of counts from a minor constituent of such a layer.
AB - Impacts of massive, highly charged glycerol clusters (≳106 Da, ≳ ± 100 charges) have been used to eject intact charged molecules of peptides, lipids, and small proteins from pure solid samples, enabling imaging using these ion species in a time-of-flight secondary ion microscope with few-micrometer spatial resolution. Here, we report mass spectra and useful ion yields (ratio of intact charged molecules detected to molecules sputtered) for several molecular species-two peptides, bradykinin and angiotensin II; two lipids, phosphatidylcholine and sphingomyelin; Irganox 1010 (a detergent); insulin; and rhodamine B-and show that useful ion yields are high enough to enable bioimaging of peptides and lipids in biological samples with few-micrometer resolution and acceptable signals. For example, several hundred molecular ion counts should be detectable from a 3 × 3 μm2 area of a pure lipid bilayer given appropriate instrumentation or tens of counts from a minor constituent of such a layer.
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U2 - 10.1021/acs.analchem.5b01802
DO - 10.1021/acs.analchem.5b01802
M3 - Article
C2 - 26452076
AN - SCOPUS:84946422914
SN - 0003-2700
VL - 87
SP - 10779
EP - 10784
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 21
ER -