Mapping the distribution of maize streak virus genotypes across the forest and transition zones of Ghana

Allen Oppong, Samuel K. Offei, Kwadwo Ofori, Hans Adu-Dapaah, Joseph N.L. Lamptey, Brigitta Kurenbach, Matthew Walters, Dionne N. Shepherd, Darren P. Martin, Arvind Varsani

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Throughout sub-Saharan Africa, maize streak virus strain A (MSV-A), the causal agent of maize streak disease (MSD), is an important biological constraint on maize production. In November/December 2010, an MSD survey was carried out in the forest and transition zones of Ghana in order to obtain MSV-A virulence sources for the development of MSD-resistant maize genotypes with agronomic properties suitable for these regions. In 79 well-distributed maize fields, the mean MSD incidence was 18.544 % and the symptom severity score was 2.956 (1 = no symptoms and 5 = extremely severe). We detected no correlation between these two variables. Phylogenetic analysis of cloned MSV-A isolates that were fully sequenced from samples collected in 51 of these fields, together with those sampled from various other parts of Africa, indicated that all of the Ghanaian isolates occurred within a broader cluster of West African isolates, all belonging to the highly virulent MSV-A1 subtype. Besides being the first report of a systematic MSV survey in Ghana, this study is the first to characterize the full-genome sequences of Ghanaian MSV isolates. The 51 genome sequences determined here will additionally be a valuable resource for the rational selection of representative MSV-A variant panels for MSD resistance screening.

Original languageEnglish (US)
Pages (from-to)483-492
Number of pages10
JournalArchives of virology
Volume160
Issue number2
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Keywords

  • Genomic
  • Ghana
  • Maize streak disease
  • Maize streak virus
  • Survey
  • Symptoms

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Mapping the distribution of maize streak virus genotypes across the forest and transition zones of Ghana'. Together they form a unique fingerprint.

  • Cite this

    Oppong, A., Offei, S. K., Ofori, K., Adu-Dapaah, H., Lamptey, J. N. L., Kurenbach, B., Walters, M., Shepherd, D. N., Martin, D. P., & Varsani, A. (2014). Mapping the distribution of maize streak virus genotypes across the forest and transition zones of Ghana. Archives of virology, 160(2), 483-492. https://doi.org/10.1007/s00705-014-2260-7