Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Sarah J R Staton, Paul V. Jones, Ginger Ku, S. Douglass Gilman, Indu Kheterpal, Mark Hayes

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Here we report a novel method for the manipulation and concentration of Aβ amyloid fibrils, implicated in Alzheimer's disease, using DC insulating gradient dielectrophoresis (DC-iGDEP). Fibril enrichment was found to be ∼400%. Simulations suggest that capture of the full range of amyloid protein aggregates is possible with optimized device design.

Original languageEnglish (US)
Pages (from-to)3227-3229
Number of pages3
JournalAnalyst
Volume137
Issue number14
DOIs
StatePublished - Jul 21 2012

Fingerprint

Equipment Design
Amyloidogenic Proteins
Electrophoresis
Amyloid
Alzheimer Disease
Monomers
protein
simulation
Protein Aggregates
method

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP). / Staton, Sarah J R; Jones, Paul V.; Ku, Ginger; Gilman, S. Douglass; Kheterpal, Indu; Hayes, Mark.

In: Analyst, Vol. 137, No. 14, 21.07.2012, p. 3227-3229.

Research output: Contribution to journalArticle

Staton, Sarah J R ; Jones, Paul V. ; Ku, Ginger ; Gilman, S. Douglass ; Kheterpal, Indu ; Hayes, Mark. / Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP). In: Analyst. 2012 ; Vol. 137, No. 14. pp. 3227-3229.
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