TY - JOUR
T1 - Manipulating degradation time in a N-isopropylacrylamide-based co-polymer with hydrolysis-dependent LCST
AU - Cui, Zhanwu
AU - Lee, Bae Hoon
AU - Pauken, Christine
AU - Vernon, Brent
N1 - Funding Information:
The authors acknowledge the National Institutes of Health (NIH) for financial support, Grant GM065917. The authors also gratefully acknowledge the use of facilities within the Center for Solid-State Science at the Arizona State University.
PY - 2010/4/1
Y1 - 2010/4/1
N2 - A thermosensitive, bioresorbable and in situ gelling co-polymer, poly(N-isopropylacrylamide-co-dimethyl-γ-butyrolactone acrylate-co-acrylic acid), was synthesized by radical co-polymerization with varying dimethyl-γ-butyrolactone acrylate (DBA) content. The materials properties were characterized using differential scanning calorimetry, gel-permeation chromatography in conjunction with static light scattering, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) and acid titration. The initial lower critical solution temperature (LCST) of the synthesized co-polymer is between room temperature and body temperature. With the increase of DBA content, the LCST decreases, but then increases after the ring-opening hydrolysis of the DBA side-group. The FT-IR and NMR spectra show the co-polymerization of three monomers, as well as the hydrolysis-dependent ring-opening of the DBA side-group. The addition of acrylic acid increases the initial LCST and accelerates the degradation rate of the co-polymer. An indirect cytotoxicity test indicated that this co-polymer has relatively low cytotoxicity as seen with 3T3 fibroblast cells.
AB - A thermosensitive, bioresorbable and in situ gelling co-polymer, poly(N-isopropylacrylamide-co-dimethyl-γ-butyrolactone acrylate-co-acrylic acid), was synthesized by radical co-polymerization with varying dimethyl-γ-butyrolactone acrylate (DBA) content. The materials properties were characterized using differential scanning calorimetry, gel-permeation chromatography in conjunction with static light scattering, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) and acid titration. The initial lower critical solution temperature (LCST) of the synthesized co-polymer is between room temperature and body temperature. With the increase of DBA content, the LCST decreases, but then increases after the ring-opening hydrolysis of the DBA side-group. The FT-IR and NMR spectra show the co-polymerization of three monomers, as well as the hydrolysis-dependent ring-opening of the DBA side-group. The addition of acrylic acid increases the initial LCST and accelerates the degradation rate of the co-polymer. An indirect cytotoxicity test indicated that this co-polymer has relatively low cytotoxicity as seen with 3T3 fibroblast cells.
KW - Bioresorbable
KW - Cytotoxicity
KW - Drug-delivery system
KW - Lower critical solution temperature
KW - Thermosensitive
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U2 - 10.1163/156856209X451323
DO - 10.1163/156856209X451323
M3 - Article
C2 - 20482992
AN - SCOPUS:77952164642
SN - 0920-5063
VL - 21
SP - 913
EP - 926
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
IS - 6-7
ER -