Manipulating degradation time in a N-isopropylacrylamide-based co-polymer with hydrolysis-dependent LCST

Zhanwu Cui, Bae Hoon Lee, Christine Pauken, Brent Vernon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A thermosensitive, bioresorbable and in situ gelling co-polymer, poly(N-isopropylacrylamide-co-dimethyl-γ-butyrolactone acrylate-co-acrylic acid), was synthesized by radical co-polymerization with varying dimethyl-γ-butyrolactone acrylate (DBA) content. The materials properties were characterized using differential scanning calorimetry, gel-permeation chromatography in conjunction with static light scattering, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) and acid titration. The initial lower critical solution temperature (LCST) of the synthesized co-polymer is between room temperature and body temperature. With the increase of DBA content, the LCST decreases, but then increases after the ring-opening hydrolysis of the DBA side-group. The FT-IR and NMR spectra show the co-polymerization of three monomers, as well as the hydrolysis-dependent ring-opening of the DBA side-group. The addition of acrylic acid increases the initial LCST and accelerates the degradation rate of the co-polymer. An indirect cytotoxicity test indicated that this co-polymer has relatively low cytotoxicity as seen with 3T3 fibroblast cells.

Original languageEnglish (US)
Pages (from-to)913-926
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume21
Issue number6-7
DOIs
StatePublished - Apr 1 2010

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Hydrolysis
Polymers
Degradation
Temperature
Cytotoxicity
Copolymerization
Acrylics
Fourier transform infrared spectroscopy
Acids
Fourier Transform Infrared Spectroscopy
Nuclear magnetic resonance
Polymerization
Magnetic Resonance Spectroscopy
Gel permeation chromatography
Fibroblasts
Titration
Light scattering
3T3 Cells
Differential scanning calorimetry
Materials properties

Keywords

  • Bioresorbable
  • Cytotoxicity
  • Drug-delivery system
  • Lower critical solution temperature
  • Thermosensitive

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Manipulating degradation time in a N-isopropylacrylamide-based co-polymer with hydrolysis-dependent LCST. / Cui, Zhanwu; Lee, Bae Hoon; Pauken, Christine; Vernon, Brent.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 21, No. 6-7, 01.04.2010, p. 913-926.

Research output: Contribution to journalArticle

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