Abstract

A groundwater containing an unusually high concentration (~4000μg/L) of perchlorate (ClO4 -) and significant (~60mg/L) sulfate (SO4 2-) was treated with hydrogen (H2)-fed biofilms. The objective was to manage the interactions between sulfate-reducing bacteria (SRB) and perchlorate-reducing bacteria (PRB) by controlling the H2-delivery capacity to achieve ClO4 - reduction to below the detection limit (4μg/L). Complete ClO4 - reduction with minimized SO4 2- reduction was achieved by using two membrane biofilm reactors (MBfRs) in series. The lead MBfR removed >96% ClO4 -, and the lag MBfR further reduced ClO4 - to below the detection limit. SO4 2- reduction ranged from 10 to 60%, and lower SO4 2- reduction corresponded to lower H2 availability (i.e., lower H2 pressure or membranes with lower H2-delivery capacity). Minimizing SO4 2- reduction improved ClO4 - removal by increasing the fraction of PRB in the biofilm. High SO4 2- flux correlated with enrichment of Desulfovibrionales, autotrophic SRB that can compete strongly with denitrifying bacteria (DB) and PRB. Increased SO4 2- reduction also led to enrichment of: 1) Ignavibacteriales and Thiobacteriales, sulfide-oxidizing bacteria that allow sulfur cycling in the biofilm; 2) Bacteroidales, heterotrophic microorganisms likely using organic sources of carbon (e.g., acetate); and 3) Spirochaetales, which potentially utilize soluble microbial products (SMPs) from autotrophic SRB to produce acetate.

Original languageEnglish (US)
Pages (from-to)215-224
Number of pages10
JournalWater Research
Volume55
DOIs
StatePublished - May 15 2014

Fingerprint

perchlorate
Groundwater
Biofilms
Sulfates
biofilm
Hydrogen
Bacteria
hydrogen
sulfate
bacterium
groundwater
sulfate-reducing bacterium
membrane
Membranes
acetate
Limit of Detection
Acetates
Spirochaetales
low pressure
Microorganisms

Keywords

  • Biofilm
  • Hydrogen
  • Perchlorate-reducing bacteria
  • Pyrosequencing
  • QPCR
  • Sulfate-reducing bacteria

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution
  • Ecological Modeling
  • Medicine(all)

Cite this

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title = "Managing the interactions between sulfate- and perchlorate-reducing bacteria when using hydrogen-fed biofilms to treat a groundwater with a high perchlorate concentration",
abstract = "A groundwater containing an unusually high concentration (~4000μg/L) of perchlorate (ClO4 -) and significant (~60mg/L) sulfate (SO4 2-) was treated with hydrogen (H2)-fed biofilms. The objective was to manage the interactions between sulfate-reducing bacteria (SRB) and perchlorate-reducing bacteria (PRB) by controlling the H2-delivery capacity to achieve ClO4 - reduction to below the detection limit (4μg/L). Complete ClO4 - reduction with minimized SO4 2- reduction was achieved by using two membrane biofilm reactors (MBfRs) in series. The lead MBfR removed >96{\%} ClO4 -, and the lag MBfR further reduced ClO4 - to below the detection limit. SO4 2- reduction ranged from 10 to 60{\%}, and lower SO4 2- reduction corresponded to lower H2 availability (i.e., lower H2 pressure or membranes with lower H2-delivery capacity). Minimizing SO4 2- reduction improved ClO4 - removal by increasing the fraction of PRB in the biofilm. High SO4 2- flux correlated with enrichment of Desulfovibrionales, autotrophic SRB that can compete strongly with denitrifying bacteria (DB) and PRB. Increased SO4 2- reduction also led to enrichment of: 1) Ignavibacteriales and Thiobacteriales, sulfide-oxidizing bacteria that allow sulfur cycling in the biofilm; 2) Bacteroidales, heterotrophic microorganisms likely using organic sources of carbon (e.g., acetate); and 3) Spirochaetales, which potentially utilize soluble microbial products (SMPs) from autotrophic SRB to produce acetate.",
keywords = "Biofilm, Hydrogen, Perchlorate-reducing bacteria, Pyrosequencing, QPCR, Sulfate-reducing bacteria",
author = "Aura Ontiveros-Valencia and Youneng Tang and Rosa Krajmalnik-Brown and Bruce Rittmann",
year = "2014",
month = "5",
day = "15",
doi = "10.1016/j.watres.2014.02.020",
language = "English (US)",
volume = "55",
pages = "215--224",
journal = "Water Research",
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TY - JOUR

T1 - Managing the interactions between sulfate- and perchlorate-reducing bacteria when using hydrogen-fed biofilms to treat a groundwater with a high perchlorate concentration

AU - Ontiveros-Valencia, Aura

AU - Tang, Youneng

AU - Krajmalnik-Brown, Rosa

AU - Rittmann, Bruce

PY - 2014/5/15

Y1 - 2014/5/15

N2 - A groundwater containing an unusually high concentration (~4000μg/L) of perchlorate (ClO4 -) and significant (~60mg/L) sulfate (SO4 2-) was treated with hydrogen (H2)-fed biofilms. The objective was to manage the interactions between sulfate-reducing bacteria (SRB) and perchlorate-reducing bacteria (PRB) by controlling the H2-delivery capacity to achieve ClO4 - reduction to below the detection limit (4μg/L). Complete ClO4 - reduction with minimized SO4 2- reduction was achieved by using two membrane biofilm reactors (MBfRs) in series. The lead MBfR removed >96% ClO4 -, and the lag MBfR further reduced ClO4 - to below the detection limit. SO4 2- reduction ranged from 10 to 60%, and lower SO4 2- reduction corresponded to lower H2 availability (i.e., lower H2 pressure or membranes with lower H2-delivery capacity). Minimizing SO4 2- reduction improved ClO4 - removal by increasing the fraction of PRB in the biofilm. High SO4 2- flux correlated with enrichment of Desulfovibrionales, autotrophic SRB that can compete strongly with denitrifying bacteria (DB) and PRB. Increased SO4 2- reduction also led to enrichment of: 1) Ignavibacteriales and Thiobacteriales, sulfide-oxidizing bacteria that allow sulfur cycling in the biofilm; 2) Bacteroidales, heterotrophic microorganisms likely using organic sources of carbon (e.g., acetate); and 3) Spirochaetales, which potentially utilize soluble microbial products (SMPs) from autotrophic SRB to produce acetate.

AB - A groundwater containing an unusually high concentration (~4000μg/L) of perchlorate (ClO4 -) and significant (~60mg/L) sulfate (SO4 2-) was treated with hydrogen (H2)-fed biofilms. The objective was to manage the interactions between sulfate-reducing bacteria (SRB) and perchlorate-reducing bacteria (PRB) by controlling the H2-delivery capacity to achieve ClO4 - reduction to below the detection limit (4μg/L). Complete ClO4 - reduction with minimized SO4 2- reduction was achieved by using two membrane biofilm reactors (MBfRs) in series. The lead MBfR removed >96% ClO4 -, and the lag MBfR further reduced ClO4 - to below the detection limit. SO4 2- reduction ranged from 10 to 60%, and lower SO4 2- reduction corresponded to lower H2 availability (i.e., lower H2 pressure or membranes with lower H2-delivery capacity). Minimizing SO4 2- reduction improved ClO4 - removal by increasing the fraction of PRB in the biofilm. High SO4 2- flux correlated with enrichment of Desulfovibrionales, autotrophic SRB that can compete strongly with denitrifying bacteria (DB) and PRB. Increased SO4 2- reduction also led to enrichment of: 1) Ignavibacteriales and Thiobacteriales, sulfide-oxidizing bacteria that allow sulfur cycling in the biofilm; 2) Bacteroidales, heterotrophic microorganisms likely using organic sources of carbon (e.g., acetate); and 3) Spirochaetales, which potentially utilize soluble microbial products (SMPs) from autotrophic SRB to produce acetate.

KW - Biofilm

KW - Hydrogen

KW - Perchlorate-reducing bacteria

KW - Pyrosequencing

KW - QPCR

KW - Sulfate-reducing bacteria

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U2 - 10.1016/j.watres.2014.02.020

DO - 10.1016/j.watres.2014.02.020

M3 - Article

VL - 55

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EP - 224

JO - Water Research

JF - Water Research

SN - 0043-1354

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