Magnetic Programmable Bead Enzyme-linked immunosorbent assay (MagProBE)

Karen Anderson (Inventor), Joshua LaBaer (Inventor)

Research output: Patent

Abstract

Innovations in proteomics have enabled the rapid discovery of potential biomarkers, such as autoantibodies, for the early detection of illnesses, including infectious diseases, autoimmune disorders and cancer. Although many biomarkers have been discovered, the high cost and time involved in validating these markers has hampered their use in clinical settings. In particular, autoantibody validation requires the assessment of selected autoantibodies amongst thousands of unrelated antibodies in sera, which is both time consuming and expensive using conventional validation tools. Researchers at the Biodesign Institute of Arizona State University have developed a rapid, inexpensive, programmable, in situ platform for display and capture of protein antigens on magnetic bead arrays for antibody detection. This platform allows for higher reproducibility and is an inexpensive tool for biomarker validation in mid-high-throughput settings. To minimize nonspecific binding of IgG to the bead arrays, a custom blocking solution was developed that further enhances the platform. Moreover, reagents and equipment that are readily available and cost-effective are used to increase the utility of this platform. This platform allows for multiplexed detection of various biomarkers in a fast and cost effective manner. It requires less patient sample, less time to prepare antigens, reduces variability between samples and has a lower cost per sample run than traditional bead-based ELISA assays. Potential Applications Biomarker validation when used in research laboratories Diagnostic and prognostic tool for various diseases when used in clinical laboratories Benefits and Advantages Small sample amount needed Higher reproducibility Cost-effective - lower cost per sample than traditional bead array ELISA assays Rapid Optimized buffers to deliver very high signal-to-noise signals Decreased epitope variability Smaller lab-bench foot-print Stable - the antibody-coupled beads have an average decrease in signal intensity of 18% for two months Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. LaBaer's laboratory webpage Dr. Anderson's laboratory webpage
Original languageEnglish (US)
StatePublished - Mar 7 2013

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Immunosorbents
Biomarkers
Assays
Enzymes
Autoantibodies
Costs
Antibodies
Clinical laboratories
Antigens
Research laboratories
Epitopes
Buffers
Immunoglobulin G
Innovation
Display devices
Throughput
Proteins

Cite this

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title = "Magnetic Programmable Bead Enzyme-linked immunosorbent assay (MagProBE)",
abstract = "Innovations in proteomics have enabled the rapid discovery of potential biomarkers, such as autoantibodies, for the early detection of illnesses, including infectious diseases, autoimmune disorders and cancer. Although many biomarkers have been discovered, the high cost and time involved in validating these markers has hampered their use in clinical settings. In particular, autoantibody validation requires the assessment of selected autoantibodies amongst thousands of unrelated antibodies in sera, which is both time consuming and expensive using conventional validation tools. Researchers at the Biodesign Institute of Arizona State University have developed a rapid, inexpensive, programmable, in situ platform for display and capture of protein antigens on magnetic bead arrays for antibody detection. This platform allows for higher reproducibility and is an inexpensive tool for biomarker validation in mid-high-throughput settings. To minimize nonspecific binding of IgG to the bead arrays, a custom blocking solution was developed that further enhances the platform. Moreover, reagents and equipment that are readily available and cost-effective are used to increase the utility of this platform. This platform allows for multiplexed detection of various biomarkers in a fast and cost effective manner. It requires less patient sample, less time to prepare antigens, reduces variability between samples and has a lower cost per sample run than traditional bead-based ELISA assays. Potential Applications Biomarker validation when used in research laboratories Diagnostic and prognostic tool for various diseases when used in clinical laboratories Benefits and Advantages Small sample amount needed Higher reproducibility Cost-effective - lower cost per sample than traditional bead array ELISA assays Rapid Optimized buffers to deliver very high signal-to-noise signals Decreased epitope variability Smaller lab-bench foot-print Stable - the antibody-coupled beads have an average decrease in signal intensity of 18{\%} for two months Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. LaBaer's laboratory webpage Dr. Anderson's laboratory webpage",
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AU - Anderson, Karen

AU - LaBaer, Joshua

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Y1 - 2013/3/7

N2 - Innovations in proteomics have enabled the rapid discovery of potential biomarkers, such as autoantibodies, for the early detection of illnesses, including infectious diseases, autoimmune disorders and cancer. Although many biomarkers have been discovered, the high cost and time involved in validating these markers has hampered their use in clinical settings. In particular, autoantibody validation requires the assessment of selected autoantibodies amongst thousands of unrelated antibodies in sera, which is both time consuming and expensive using conventional validation tools. Researchers at the Biodesign Institute of Arizona State University have developed a rapid, inexpensive, programmable, in situ platform for display and capture of protein antigens on magnetic bead arrays for antibody detection. This platform allows for higher reproducibility and is an inexpensive tool for biomarker validation in mid-high-throughput settings. To minimize nonspecific binding of IgG to the bead arrays, a custom blocking solution was developed that further enhances the platform. Moreover, reagents and equipment that are readily available and cost-effective are used to increase the utility of this platform. This platform allows for multiplexed detection of various biomarkers in a fast and cost effective manner. It requires less patient sample, less time to prepare antigens, reduces variability between samples and has a lower cost per sample run than traditional bead-based ELISA assays. Potential Applications Biomarker validation when used in research laboratories Diagnostic and prognostic tool for various diseases when used in clinical laboratories Benefits and Advantages Small sample amount needed Higher reproducibility Cost-effective - lower cost per sample than traditional bead array ELISA assays Rapid Optimized buffers to deliver very high signal-to-noise signals Decreased epitope variability Smaller lab-bench foot-print Stable - the antibody-coupled beads have an average decrease in signal intensity of 18% for two months Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. LaBaer's laboratory webpage Dr. Anderson's laboratory webpage

AB - Innovations in proteomics have enabled the rapid discovery of potential biomarkers, such as autoantibodies, for the early detection of illnesses, including infectious diseases, autoimmune disorders and cancer. Although many biomarkers have been discovered, the high cost and time involved in validating these markers has hampered their use in clinical settings. In particular, autoantibody validation requires the assessment of selected autoantibodies amongst thousands of unrelated antibodies in sera, which is both time consuming and expensive using conventional validation tools. Researchers at the Biodesign Institute of Arizona State University have developed a rapid, inexpensive, programmable, in situ platform for display and capture of protein antigens on magnetic bead arrays for antibody detection. This platform allows for higher reproducibility and is an inexpensive tool for biomarker validation in mid-high-throughput settings. To minimize nonspecific binding of IgG to the bead arrays, a custom blocking solution was developed that further enhances the platform. Moreover, reagents and equipment that are readily available and cost-effective are used to increase the utility of this platform. This platform allows for multiplexed detection of various biomarkers in a fast and cost effective manner. It requires less patient sample, less time to prepare antigens, reduces variability between samples and has a lower cost per sample run than traditional bead-based ELISA assays. Potential Applications Biomarker validation when used in research laboratories Diagnostic and prognostic tool for various diseases when used in clinical laboratories Benefits and Advantages Small sample amount needed Higher reproducibility Cost-effective - lower cost per sample than traditional bead array ELISA assays Rapid Optimized buffers to deliver very high signal-to-noise signals Decreased epitope variability Smaller lab-bench foot-print Stable - the antibody-coupled beads have an average decrease in signal intensity of 18% for two months Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. LaBaer's laboratory webpage Dr. Anderson's laboratory webpage

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