Magnetic Bead Characterization, Implementation and Control for the Extraction of Nucleic Acids from Patient Biofluid Samples in Point of Need Microfluidic Devices

Shilpita Biswas, Siril Arockiam, Jennifer M.Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The application of microfluidics in Point of Care (PoC) testing was a breakthrough in PoC diagnostics, yet high precision testing remains a challenge. In order to extract and isolate pure RNA or DNA from biofluids containing a virus or virion, small spherical magnetic particles called magnetic beads are commonly used in laboratories. Magnetic beads have many applications ranging from purification of nucleic acids, magnetic cell separation, targeted drug delivery to brain or malignant tumors, magnetic chaining etc. Unfortunately, integrating magnetic beads into PoC devices is challenging. This paper delves into a comparative study of the behaviour and parameters of five different sizes of carboxyl coated paramagnetic beads along with silica coated dynabeads. Their behavior is characterized in a microcapillary channel for a SARS-CoV-2 virus PoC testing kit to enrich the sample, concentrating the nucleic acid. This step would increase the sensitivity and specificity for any nucleic acid-based PoC testing device.

Original languageEnglish (US)
Title of host publication2021 IEEE International Midwest Symposium on Circuits and Systems, MWSCAS 2021 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages408-411
Number of pages4
ISBN (Electronic)9781665424615
DOIs
StatePublished - Aug 9 2021
Event2021 IEEE International Midwest Symposium on Circuits and Systems, MWSCAS 2021 - Virtual, East Lansing, United States
Duration: Aug 9 2021Aug 11 2021

Publication series

NameMidwest Symposium on Circuits and Systems
Volume2021-August
ISSN (Print)1548-3746

Conference

Conference2021 IEEE International Midwest Symposium on Circuits and Systems, MWSCAS 2021
Country/TerritoryUnited States
CityVirtual, East Lansing
Period8/9/218/11/21

Keywords

  • electromagnets
  • magnetic beads
  • MEMS
  • microfluidics
  • nucleic acids
  • paramagnetic
  • permanent magnets
  • point-of-care
  • superparamagnetic

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

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