M1 RNA with large terminal deletions retains its catalytic activity

Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Truncated transcripts of the rnpB gene from E. coli, coding for M1 RNA, the catalytic subunit of RNAase P, and fragments of M1 RNA generated by nuclease treatment have been prepared, and their ability to function catalytically in vitro has been determined. Molecules missing as many as 122 nucleotides at the 3′ terminus retain catalytic activity, although at a much lower level than M1 RNA itself, No activity is observed with an RNA that is missing 70 nucleotides at the 5′ terminus. The removal of even a small number of nucleotides from both termini eliminates all catalytic function. The preservation of one intact terminus may be essential for the tertiary and quaternary interactions required to generate the conformation of an active RNA species.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalCell
Volume45
Issue number2
DOIs
StatePublished - Apr 25 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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