M1 RNA, the RNA Subunit of Escherichia coli Ribonuclease P, Can Undergo a pH-Sensitive Conformational Change

Sidney Altman, Cecilia Guerrier-Takada

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

After purification from extracts of whole cells. Ml RNA, the catalytic subunit of ribonuclease P from Escherichia coli, apparently must undergo a change in conformation before it can function catalytically. The rate of this conformational change is dependent upon the duration of incubation at various temperatures and pH. ΔE* of the transition at pH 7.5 is approximately 36 kcal/mol. The change in conformation is not sensitive to Mg2+ concentration between 10 and 100 mM. A decrease in A260 of Ml RNA in solution has been observed during the incubation period that potentiates the conformational change at 30 °C, but no direct correlation can yet be made to specific structural rearrangements.

Original languageEnglish (US)
Pages (from-to)1205-1208
Number of pages4
JournalBiochemistry
Volume25
Issue number6
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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