Lumen thiol oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis

Mohamed Karamoko, Sara Cline, Kevin Redding, Natividad Ruiz, Patrice P. Hamel

    Research output: Contribution to journalArticle

    55 Citations (Scopus)

    Abstract

    Here, we identify Arabidopsis thaliana Lumen Thiol Oxidoreductase1 (LTO1) as a disulfide bond-forming enzyme in the thylakoid lumen. Using topological reporters in bacteria, we deduced a lumenal location for the redox active domains of the protein. LTO1 can partially substitute for the proteins catalyzing disulfide bond formation in the bacterial periplasm, which is topologically equivalent to the plastid lumen. An insertional mutation within the LTO1 promoter is associated with a severe photoautotrophic growth defect. Measurements of the photosynthetic activity indicate that the lto1 mutant displays a limitation in the electron flow from photosystem II (PSII). In accordance with these measurements, we noted a severe depletion of the structural subunits of PSII but no change in the accumulation of the cytochrome b 6f complex or photosystem I. In a yeast two-hybrid assay, the thioredoxin-like domain of LTO1 interacts with PsbO, a lumenal PSII subunit known to be disulfide bonded, and a recombinant form of the molecule can introduce a disulfide bond in PsbO in vitro. The documentation of a sulfhydryl-oxidizing activity in the thylakoid lumen further underscores the importance of catalyzed thiol-disulfide chemistry for the biogenesis of the thylakoid compartment.

    Original languageEnglish (US)
    Pages (from-to)4462-4475
    Number of pages14
    JournalPlant Cell
    Volume23
    Issue number12
    DOIs
    StatePublished - Dec 2011

    Fingerprint

    Photosystem II Protein Complex
    disulfide bonds
    thiols
    catalysts
    Arabidopsis
    Sulfhydryl Compounds
    Disulfides
    photosystem II
    Thylakoids
    thylakoids
    sulfides
    NADH dehydrogenase (ubiquinone)
    Photosystem I Protein Complex
    Periplasm
    two hybrid system techniques
    Two-Hybrid System Techniques
    Thioredoxins
    Cytochromes b
    Plastids
    photosystem I

    ASJC Scopus subject areas

    • Plant Science
    • Cell Biology

    Cite this

    Lumen thiol oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis. / Karamoko, Mohamed; Cline, Sara; Redding, Kevin; Ruiz, Natividad; Hamel, Patrice P.

    In: Plant Cell, Vol. 23, No. 12, 12.2011, p. 4462-4475.

    Research output: Contribution to journalArticle

    Karamoko, Mohamed ; Cline, Sara ; Redding, Kevin ; Ruiz, Natividad ; Hamel, Patrice P. / Lumen thiol oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis. In: Plant Cell. 2011 ; Vol. 23, No. 12. pp. 4462-4475.
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