Lumen thiol oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis

Mohamed Karamoko, Sara Cline, Kevin Redding, Natividad Ruiz, Patrice P. Hamel

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Here, we identify Arabidopsis thaliana Lumen Thiol Oxidoreductase1 (LTO1) as a disulfide bond-forming enzyme in the thylakoid lumen. Using topological reporters in bacteria, we deduced a lumenal location for the redox active domains of the protein. LTO1 can partially substitute for the proteins catalyzing disulfide bond formation in the bacterial periplasm, which is topologically equivalent to the plastid lumen. An insertional mutation within the LTO1 promoter is associated with a severe photoautotrophic growth defect. Measurements of the photosynthetic activity indicate that the lto1 mutant displays a limitation in the electron flow from photosystem II (PSII). In accordance with these measurements, we noted a severe depletion of the structural subunits of PSII but no change in the accumulation of the cytochrome b 6f complex or photosystem I. In a yeast two-hybrid assay, the thioredoxin-like domain of LTO1 interacts with PsbO, a lumenal PSII subunit known to be disulfide bonded, and a recombinant form of the molecule can introduce a disulfide bond in PsbO in vitro. The documentation of a sulfhydryl-oxidizing activity in the thylakoid lumen further underscores the importance of catalyzed thiol-disulfide chemistry for the biogenesis of the thylakoid compartment.

Original languageEnglish (US)
Pages (from-to)4462-4475
Number of pages14
JournalPlant Cell
Volume23
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Photosystem II Protein Complex
disulfide bonds
thiols
catalysts
Arabidopsis
Sulfhydryl Compounds
Disulfides
photosystem II
Thylakoids
thylakoids
sulfides
NADH dehydrogenase (ubiquinone)
Photosystem I Protein Complex
Periplasm
two hybrid system techniques
Two-Hybrid System Techniques
Thioredoxins
Cytochromes b
Plastids
photosystem I

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Lumen thiol oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis. / Karamoko, Mohamed; Cline, Sara; Redding, Kevin; Ruiz, Natividad; Hamel, Patrice P.

In: Plant Cell, Vol. 23, No. 12, 12.2011, p. 4462-4475.

Research output: Contribution to journalArticle

Karamoko, Mohamed ; Cline, Sara ; Redding, Kevin ; Ruiz, Natividad ; Hamel, Patrice P. / Lumen thiol oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis. In: Plant Cell. 2011 ; Vol. 23, No. 12. pp. 4462-4475.
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