Lower Fasted-State but Greater Increase in Muscle Protein Synthesis in Response to Elevated Plasma Amino Acids in Obesity

Lee Tran, Katon A. Kras, Nyssa Hoffman, Jayachandran Ravichandran, Jared Dickinson, Andrew D'Lugos, Chad C. Carroll, Shivam H. Patel, Lawrence J. Mandarino, Lori Roust, Christos Katsanos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: Obesity alters protein metabolism in skeletal muscle, but consistent evidence is lacking. This study compared muscle protein synthesis in adults with obesity and in lean controls in the fasted state and during an amino acid infusion. Methods: Ten subjects with obesity (age: 36 ± 3 years; BMI: 34 ± 1 kg/m2) and ten controls (age: 35 ± 3 years; BMI: 23 ± 1 kg/m2) received an infusion of L-[2,3,3,4,5,5,5,6,6,6-2H10]leucine (0.15 μmol/kg fat-free mass/min) to measure muscle protein synthesis after an overnight fast and during amino acid infusion. Results: Despite greater muscle mammalian target of rapamycin phosphorylation (P ≤ 0.05), fasted-state mixed-muscle and mitochondrial protein synthesis were lower in subjects with obesity (P ≤ 0.05). However, the change in mixed-muscle protein synthesis during the amino acid infusion was 2.7-fold greater in subjects with obesity (P ≤ 0.05), accompanied by a greater change in S6 kinase-1 phosphorylation (P ≤ 0.05). The change in mitochondrial protein synthesis did not differ between groups (P > 0.05). Conclusions: Adults with obesity have reduced muscle protein synthesis in the fasted state, but this response is compensated for by a greater change in overall muscle protein synthesis during amino acid infusion.

Original languageEnglish (US)
Pages (from-to)1179-1187
Number of pages9
JournalObesity
Volume26
Issue number7
DOIs
StatePublished - Jul 1 2018

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Muscle Proteins
Obesity
Amino Acids
Mitochondrial Proteins
Phosphorylation
Ribosomal Protein S6 Kinases
Sirolimus
Leucine
Skeletal Muscle
Fats
Muscles
Proteins

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

Lower Fasted-State but Greater Increase in Muscle Protein Synthesis in Response to Elevated Plasma Amino Acids in Obesity. / Tran, Lee; Kras, Katon A.; Hoffman, Nyssa; Ravichandran, Jayachandran; Dickinson, Jared; D'Lugos, Andrew; Carroll, Chad C.; Patel, Shivam H.; Mandarino, Lawrence J.; Roust, Lori; Katsanos, Christos.

In: Obesity, Vol. 26, No. 7, 01.07.2018, p. 1179-1187.

Research output: Contribution to journalArticle

Tran, L, Kras, KA, Hoffman, N, Ravichandran, J, Dickinson, J, D'Lugos, A, Carroll, CC, Patel, SH, Mandarino, LJ, Roust, L & Katsanos, C 2018, 'Lower Fasted-State but Greater Increase in Muscle Protein Synthesis in Response to Elevated Plasma Amino Acids in Obesity', Obesity, vol. 26, no. 7, pp. 1179-1187. https://doi.org/10.1002/oby.22213
Tran, Lee ; Kras, Katon A. ; Hoffman, Nyssa ; Ravichandran, Jayachandran ; Dickinson, Jared ; D'Lugos, Andrew ; Carroll, Chad C. ; Patel, Shivam H. ; Mandarino, Lawrence J. ; Roust, Lori ; Katsanos, Christos. / Lower Fasted-State but Greater Increase in Muscle Protein Synthesis in Response to Elevated Plasma Amino Acids in Obesity. In: Obesity. 2018 ; Vol. 26, No. 7. pp. 1179-1187.
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