Low-shear modeled microgravity: A global environmental regulatory signal affecting bacterial gene expression, physiology, and pathogenesis

Cheryl Nickerson, C. Mark Ott, James W. Wilson, Rajee Ramamurthy, Carly L. LeBlanc, Kerstin Höner zu Bentrup, Timothy Hammond, Duane L. Pierson

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Bacteria inhabit an impressive variety of ecological niches and must adapt constantly to changing environmental conditions. While numerous environmental signals have been examined for their effect on bacteria, the effects of mechanical forces such as shear stress and gravity have only been investigated to a limited extent. However, several important studies have demonstrated a key role for the environmental signals of low shear and/or microgravity in the regulation of bacterial gene expression, physiology, and pathogenesis [Chem. Rec. 1 (2001) 333; Appl. Microbiol. Biotechnol. 54 (2000) 33; Appl. Environ. Microbiol. 63 (1997) 4090; J. Ind. Microbiol. 18 (1997) 22; Curr. Microbiol. 34(4) (1997) 199; Appl. Microbiol. Biotechnol. 56(3-4) (2001) 384; Infect Immun. 68(6) (2000) 3147; Cell 109(7) (2002) 913; Appl. Environ. Microbiol. 68(11) (2002) 5408; Proc. Natl. Acad. Sci. U. S. A. 99(21) (2002) 13807]. The response of bacteria to these environmental signals, which are similar to those encountered during prokaryotic life cycles, may provide insight into bacterial adaptations to physiologically relevant conditions. This review focuses on the current and potential future research trends aimed at understanding the effect of the mechanical forces of low shear and microgravity analogues on different bacterial parameters. In addition, this review also discusses the use of microgravity technology to generate physiologically relevant human tissue models for research in bacterial pathogenesis.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalJournal of Microbiological Methods
Volume54
Issue number1
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Bacterial Genes
Weightlessness
Bacteria
Gene Expression
Bacterial Gene Expression Regulation
Gravitation
Life Cycle Stages
Technology
Research
Environ

Keywords

  • Bacteria
  • Bioreactor
  • Gene expression
  • Low shear
  • Modeled microgravity
  • Optimized suspension culture
  • Pathogenesis
  • Physiology
  • Rotating wall vessel

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Low-shear modeled microgravity : A global environmental regulatory signal affecting bacterial gene expression, physiology, and pathogenesis. / Nickerson, Cheryl; Ott, C. Mark; Wilson, James W.; Ramamurthy, Rajee; LeBlanc, Carly L.; Höner zu Bentrup, Kerstin; Hammond, Timothy; Pierson, Duane L.

In: Journal of Microbiological Methods, Vol. 54, No. 1, 01.07.2003, p. 1-11.

Research output: Contribution to journalArticle

Nickerson, Cheryl ; Ott, C. Mark ; Wilson, James W. ; Ramamurthy, Rajee ; LeBlanc, Carly L. ; Höner zu Bentrup, Kerstin ; Hammond, Timothy ; Pierson, Duane L. / Low-shear modeled microgravity : A global environmental regulatory signal affecting bacterial gene expression, physiology, and pathogenesis. In: Journal of Microbiological Methods. 2003 ; Vol. 54, No. 1. pp. 1-11.
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