Abstract
The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.
| Original language | English (US) |
|---|---|
| Title of host publication | 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 154-157 |
| Number of pages | 4 |
| ISBN (Electronic) | 9781509011667 |
| DOIs | |
| State | Published - Dec 27 2016 |
| Event | 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 - Cancun, Mexico Duration: Nov 9 2016 → Nov 11 2016 |
Other
| Other | 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 |
|---|---|
| Country | Mexico |
| City | Cancun |
| Period | 11/9/16 → 11/11/16 |
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ASJC Scopus subject areas
- Health Informatics
- Medicine (miscellaneous)
Cite this
Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics. / Smith, Joseph T.; Obahiagbon, Uwadiae; Ewaisha, Radwa; Katchman, Benjamin A.; Kaftanoglu, Korhan; Arafa, Hany M.; Kullman, Dixie E.; Anderson, Karen; Blain Christen, Jennifer.
2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 154-157 7797720.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics
AU - Smith, Joseph T.
AU - Obahiagbon, Uwadiae
AU - Ewaisha, Radwa
AU - Katchman, Benjamin A.
AU - Kaftanoglu, Korhan
AU - Arafa, Hany M.
AU - Kullman, Dixie E.
AU - Anderson, Karen
AU - Blain Christen, Jennifer
PY - 2016/12/27
Y1 - 2016/12/27
N2 - The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.
AB - The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.
UR - http://www.scopus.com/inward/record.url?scp=85010641842&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010641842&partnerID=8YFLogxK
U2 - 10.1109/HIC.2016.7797720
DO - 10.1109/HIC.2016.7797720
M3 - Conference contribution
AN - SCOPUS:85010641842
SP - 154
EP - 157
BT - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
PB - Institute of Electrical and Electronics Engineers Inc.
ER -