Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics

Joseph T. Smith, Uwadiae Obahiagbon, Radwa Ewaisha, Benjamin A. Katchman, Korhan Kaftanoglu, Hany M. Arafa, Dixie E. Kullman, Karen Anderson, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.

Original languageEnglish (US)
Title of host publication2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages154-157
Number of pages4
ISBN (Electronic)9781509011667
DOIs
StatePublished - Dec 27 2016
Event2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 - Cancun, Mexico
Duration: Nov 9 2016Nov 11 2016

Other

Other2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
CountryMexico
CityCancun
Period11/9/1611/11/16

ASJC Scopus subject areas

  • Health Informatics
  • Medicine (miscellaneous)

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    Smith, J. T., Obahiagbon, U., Ewaisha, R., Katchman, B. A., Kaftanoglu, K., Arafa, H. M., Kullman, D. E., Anderson, K., & Blain Christen, J. (2016). Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics. In 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 (pp. 154-157). [7797720] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HIC.2016.7797720