Abstract
The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.
Original language | English (US) |
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Title of host publication | 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 |
Publisher | Institute of Electrical and Electronics Engineers Inc. |
Pages | 154-157 |
Number of pages | 4 |
ISBN (Electronic) | 9781509011667 |
DOIs | |
State | Published - Dec 27 2016 |
Event | 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 - Cancun, Mexico Duration: Nov 9 2016 → Nov 11 2016 |
Other
Other | 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 |
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Country | Mexico |
City | Cancun |
Period | 11/9/16 → 11/11/16 |
ASJC Scopus subject areas
- Health Informatics
- Medicine (miscellaneous)