Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics

Joseph T. Smith, Uwadiae Obahiagbon, Radwa Ewaisha, Benjamin A. Katchman, Korhan Kaftanoglu, Hany M. Arafa, Dixie E. Kullman, Karen Anderson, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples. Our approach combines an inexpensive 4-site microscope slide reader with low per patient cost consumables using conventional glass microscope slides, pre-printed with biorecognition molecules. We use a neuromorphic-inspired, charge-integrating readout circuit architecture combined with optical filters in a sandwich-style configuration to achieve high-sensitivity while trading off detection time and number of biorecognition sites per slide. Our demonstrated lower limit of detection using Nile Red fluorescent microspheres was a 1,000,000:1 dilution corresponding to approximately 200 microspheres per detection site, approaching the sensitivity of a conventional high-cost desktop clinical laboratory microscope slide reader.

Original languageEnglish (US)
Title of host publication2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages154-157
Number of pages4
ISBN (Electronic)9781509011667
DOIs
StatePublished - Dec 27 2016
Event2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 - Cancun, Mexico
Duration: Nov 9 2016Nov 11 2016

Other

Other2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
CountryMexico
CityCancun
Period11/9/1611/11/16

Fingerprint

Point-of-Care Systems
Molecular Pathology
Fluorescence
Costs and Cost Analysis
Microspheres
Glass
Limit of Detection
Technology
Serum

ASJC Scopus subject areas

  • Health Informatics
  • Medicine (miscellaneous)

Cite this

Smith, J. T., Obahiagbon, U., Ewaisha, R., Katchman, B. A., Kaftanoglu, K., Arafa, H. M., ... Blain Christen, J. (2016). Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics. In 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016 (pp. 154-157). [7797720] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HIC.2016.7797720

Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics. / Smith, Joseph T.; Obahiagbon, Uwadiae; Ewaisha, Radwa; Katchman, Benjamin A.; Kaftanoglu, Korhan; Arafa, Hany M.; Kullman, Dixie E.; Anderson, Karen; Blain Christen, Jennifer.

2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 154-157 7797720.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Smith, JT, Obahiagbon, U, Ewaisha, R, Katchman, BA, Kaftanoglu, K, Arafa, HM, Kullman, DE, Anderson, K & Blain Christen, J 2016, Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics. in 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016., 7797720, Institute of Electrical and Electronics Engineers Inc., pp. 154-157, 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016, Cancun, Mexico, 11/9/16. https://doi.org/10.1109/HIC.2016.7797720
Smith JT, Obahiagbon U, Ewaisha R, Katchman BA, Kaftanoglu K, Arafa HM et al. Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics. In 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 154-157. 7797720 https://doi.org/10.1109/HIC.2016.7797720
Smith, Joseph T. ; Obahiagbon, Uwadiae ; Ewaisha, Radwa ; Katchman, Benjamin A. ; Kaftanoglu, Korhan ; Arafa, Hany M. ; Kullman, Dixie E. ; Anderson, Karen ; Blain Christen, Jennifer. / Low-cost, disposable fluorescence-based biorecognition system architecture for multiplexed point-of-care molecular diagnostics. 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 154-157
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