Abstract
Glutathione S-transferase (GST) μ phenotype was assessed in colon tissue from patients with ulcerative colitis and colorectal neoplasms that were positive for GSTM1 genotype. GST μ protein (enzyme linked immunosorbent assay) was absent in 2/9 unaffected colon tissue (22.3%), 4/13 tissues with chronic ulcerative colitis (CUC) (30.7%), 4/11 adenomas (36.4%) and 7/14 adenocarcinomas (50.0%; P≤0.05). GST-3H-trans-stilbene oxide activity was detected in all tissues except 1/13 (7.6%) CUC tissues and 7/14 (50.0%, P≤0.05) colorectal adenocarcinomas. Immunoreactive GST μ was observed in colonic epithelial cells but not in adjacent neoplastic cells by immunohistochemistry. Two-dimensional electrophoresis revealed several μ class isoforms in cytosol from unaffected colon that were absent in matched tumor cytosol. These results indicate that GSTM1 genotype may not necessarily reflect GST μ phenotype in colorectal tumors.
Original language | English (US) |
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Pages (from-to) | 65-74 |
Number of pages | 10 |
Journal | Cancer Letters |
Volume | 177 |
Issue number | 1 |
DOIs | |
State | Published - Mar 8 2002 |
Externally published | Yes |
Keywords
- Colon
- Genotype
- Glutathione S-transferase
- Phenotype
ASJC Scopus subject areas
- Oncology
- Cancer Research