Loss of Fnip1 alters kidney developmental transcriptional program and synergizes with TSC1 loss to promote mTORC1 activation and renal cyst formation

Ryan Centini, Mark Tsang, Terri Iwata, Heon Park, Jeffrey Delrow, Daciana Margineantu, Brandon M. Iritani, Haiwei Gu, H. Denny Liggitt, Janella Kang, Lim Kang, David M. Hockenbery, Daniel Raftery, Brian M. Iritani

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Birt-Hogg-Dube' Syndrome (BHDS) is a rare genetic disorder in humans characterized by skin hamartomas, lung cysts, pneumothorax, and increased risk of renal tumors. BHDS is caused by mutations in the BHD gene, which encodes for Folliculin, a cytoplasmic adapter protein that binds to Folliculin interacting proteins-1 and-2 (Fnip1, Fnip2) as well as the master energy sensor AMP kinase (AMPK). Whereas kidney-specific deletion of the Bhd gene in mice is known to result in polycystic kidney disease (PKD) and renal cell carcinoma, the roles of Fnip1 in renal cell development and function are unclear. In this study, we utilized mice with constitutive deletion of the Fnip1 gene to show that the loss of Fnip1 is sufficient to result in renal cyst formation, which was characterized by decreased AMPK activation, increased mTOR activation, and metabolic hyperactivation. Using RNAseq, we found that Fnip1 disruption resulted in many cellular and molecular changes previously implicated in the development of PKD in humans, including alterations in the expression of ion and amino acid transporters, increased cell adhesion, and increased inflammation. Loss of Fnip1 synergized with Tsc1 loss to hyperactivate mTOR, increase Erk activation, and greatly accelerate the development of PKD. Our results collectively define roles for Fnip1 in regulating kidney development and function, and provide a model for how loss of Fnip1 contributes to PKD and perhaps renal cell carcinoma.

    Original languageEnglish (US)
    Article number0197973
    JournalPLoS One
    Volume13
    Issue number6
    DOIs
    StatePublished - Jun 1 2018

    Fingerprint

    Polycystic Kidney Diseases
    Cysts
    kidney cells
    Chemical activation
    kidneys
    Birt-Hogg-Dube Syndrome
    Kidney
    Adenylate Kinase
    AMP-activated protein kinase
    estrone
    gene deletion
    Estrone
    Genes
    carcinoma
    Gene Deletion
    Renal Cell Carcinoma
    Cells
    hamartoma
    pneumothorax
    amino acid transporters

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

    Loss of Fnip1 alters kidney developmental transcriptional program and synergizes with TSC1 loss to promote mTORC1 activation and renal cyst formation. / Centini, Ryan; Tsang, Mark; Iwata, Terri; Park, Heon; Delrow, Jeffrey; Margineantu, Daciana; Iritani, Brandon M.; Gu, Haiwei; Liggitt, H. Denny; Kang, Janella; Kang, Lim; Hockenbery, David M.; Raftery, Daniel; Iritani, Brian M.

    In: PLoS One, Vol. 13, No. 6, 0197973, 01.06.2018.

    Research output: Contribution to journalArticle

    Centini, R, Tsang, M, Iwata, T, Park, H, Delrow, J, Margineantu, D, Iritani, BM, Gu, H, Liggitt, HD, Kang, J, Kang, L, Hockenbery, DM, Raftery, D & Iritani, BM 2018, 'Loss of Fnip1 alters kidney developmental transcriptional program and synergizes with TSC1 loss to promote mTORC1 activation and renal cyst formation', PLoS One, vol. 13, no. 6, 0197973. https://doi.org/10.1371/journal.pone.0197973
    Centini, Ryan ; Tsang, Mark ; Iwata, Terri ; Park, Heon ; Delrow, Jeffrey ; Margineantu, Daciana ; Iritani, Brandon M. ; Gu, Haiwei ; Liggitt, H. Denny ; Kang, Janella ; Kang, Lim ; Hockenbery, David M. ; Raftery, Daniel ; Iritani, Brian M. / Loss of Fnip1 alters kidney developmental transcriptional program and synergizes with TSC1 loss to promote mTORC1 activation and renal cyst formation. In: PLoS One. 2018 ; Vol. 13, No. 6.
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    AU - Iwata, Terri

    AU - Park, Heon

    AU - Delrow, Jeffrey

    AU - Margineantu, Daciana

    AU - Iritani, Brandon M.

    AU - Gu, Haiwei

    AU - Liggitt, H. Denny

    AU - Kang, Janella

    AU - Kang, Lim

    AU - Hockenbery, David M.

    AU - Raftery, Daniel

    AU - Iritani, Brian M.

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