Longitudinal variation of microbial communities in benthic biofilms and association with hydrological and physicochemical conditions in glacier-fed streams

Ze Ren, Hongkai Gao, James Elser

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Glacier-fed streams are highly dynamic environments that integrate upstream catchment processes and are prominent geomorphological and ecological components of alpine landscapes. In these systems, hydrological and physicochemical factors change significantly with location downstream of the glacier. Variation in microbial communities in benthic biofilms along such gradients are not well studied, nor do we understand how hydrological and physicochemical factors drive those changes. We characterized microbial community structure in 2 glacier-fed streams in the Tianshan Mountains, central Asia, by sequencing 16S ribosomal (r)RNA genes in benthic biofilms and documented abiotic environmental conditions. Alpha diversity indices of microbes in benthic biofilms (number of operational taxonomic units, evenness, phylogenetic diversity) were significantly related to hydrological factors, including distance to glacier (GD), glacier area proportion (GA), and glacier source proportion (GS), and physicochemical factors, including water temperature, pH, dissolved organic C (DOC), total N (TN), and NO3. The dominant phyla were Proteobacteria (46% of operational taxonomic units [OTUs]), Cyanobacteria (16%), Bacteroidetes (12%), Actinobacteria (9%), and Acidobacteria (6%). Microbial communities differed longitudinally along the stream at the OTU level and even at the phylum level. Correlation, canonical correlation, and network analyses showed that the microbes had significant associations with hydrological (GA, GS, and GD), biogeochemical (TN, NO3, DOC, total P, and soluble reactive P), and physicochemical (pH) factors. These results add to our knowledge of microbial community structure and potential drivers of that structure in glacier-fed stream ecosystems and provide potentially valuable data for assessing future dynamics as these systems experience further disruption caused by the influences of climate change.

Original languageEnglish (US)
Pages (from-to)479-490
Number of pages12
JournalFreshwater Science
Volume36
Issue number3
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

Fingerprint

glaciers
biofilm
microbial communities
microbial community
glacier
community structure
hydrologic factors
microorganisms
Acidobacteria
dissolved organic nitrogen
Actinobacteria
Central Asia
Proteobacteria
diversity index
RNA
Cyanobacteria
cyanobacterium
water temperature
environmental conditions
mountains

Keywords

  • Benthic biofilm
  • Glacier-fed streams
  • Hydrology
  • Microbial community
  • Microbial diversity
  • Physicochemical factors

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Aquatic Science

Cite this

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title = "Longitudinal variation of microbial communities in benthic biofilms and association with hydrological and physicochemical conditions in glacier-fed streams",
abstract = "Glacier-fed streams are highly dynamic environments that integrate upstream catchment processes and are prominent geomorphological and ecological components of alpine landscapes. In these systems, hydrological and physicochemical factors change significantly with location downstream of the glacier. Variation in microbial communities in benthic biofilms along such gradients are not well studied, nor do we understand how hydrological and physicochemical factors drive those changes. We characterized microbial community structure in 2 glacier-fed streams in the Tianshan Mountains, central Asia, by sequencing 16S ribosomal (r)RNA genes in benthic biofilms and documented abiotic environmental conditions. Alpha diversity indices of microbes in benthic biofilms (number of operational taxonomic units, evenness, phylogenetic diversity) were significantly related to hydrological factors, including distance to glacier (GD), glacier area proportion (GA), and glacier source proportion (GS), and physicochemical factors, including water temperature, pH, dissolved organic C (DOC), total N (TN), and NO3. The dominant phyla were Proteobacteria (46{\%} of operational taxonomic units [OTUs]), Cyanobacteria (16{\%}), Bacteroidetes (12{\%}), Actinobacteria (9{\%}), and Acidobacteria (6{\%}). Microbial communities differed longitudinally along the stream at the OTU level and even at the phylum level. Correlation, canonical correlation, and network analyses showed that the microbes had significant associations with hydrological (GA, GS, and GD), biogeochemical (TN, NO3, DOC, total P, and soluble reactive P), and physicochemical (pH) factors. These results add to our knowledge of microbial community structure and potential drivers of that structure in glacier-fed stream ecosystems and provide potentially valuable data for assessing future dynamics as these systems experience further disruption caused by the influences of climate change.",
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T1 - Longitudinal variation of microbial communities in benthic biofilms and association with hydrological and physicochemical conditions in glacier-fed streams

AU - Ren, Ze

AU - Gao, Hongkai

AU - Elser, James

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N2 - Glacier-fed streams are highly dynamic environments that integrate upstream catchment processes and are prominent geomorphological and ecological components of alpine landscapes. In these systems, hydrological and physicochemical factors change significantly with location downstream of the glacier. Variation in microbial communities in benthic biofilms along such gradients are not well studied, nor do we understand how hydrological and physicochemical factors drive those changes. We characterized microbial community structure in 2 glacier-fed streams in the Tianshan Mountains, central Asia, by sequencing 16S ribosomal (r)RNA genes in benthic biofilms and documented abiotic environmental conditions. Alpha diversity indices of microbes in benthic biofilms (number of operational taxonomic units, evenness, phylogenetic diversity) were significantly related to hydrological factors, including distance to glacier (GD), glacier area proportion (GA), and glacier source proportion (GS), and physicochemical factors, including water temperature, pH, dissolved organic C (DOC), total N (TN), and NO3. The dominant phyla were Proteobacteria (46% of operational taxonomic units [OTUs]), Cyanobacteria (16%), Bacteroidetes (12%), Actinobacteria (9%), and Acidobacteria (6%). Microbial communities differed longitudinally along the stream at the OTU level and even at the phylum level. Correlation, canonical correlation, and network analyses showed that the microbes had significant associations with hydrological (GA, GS, and GD), biogeochemical (TN, NO3, DOC, total P, and soluble reactive P), and physicochemical (pH) factors. These results add to our knowledge of microbial community structure and potential drivers of that structure in glacier-fed stream ecosystems and provide potentially valuable data for assessing future dynamics as these systems experience further disruption caused by the influences of climate change.

AB - Glacier-fed streams are highly dynamic environments that integrate upstream catchment processes and are prominent geomorphological and ecological components of alpine landscapes. In these systems, hydrological and physicochemical factors change significantly with location downstream of the glacier. Variation in microbial communities in benthic biofilms along such gradients are not well studied, nor do we understand how hydrological and physicochemical factors drive those changes. We characterized microbial community structure in 2 glacier-fed streams in the Tianshan Mountains, central Asia, by sequencing 16S ribosomal (r)RNA genes in benthic biofilms and documented abiotic environmental conditions. Alpha diversity indices of microbes in benthic biofilms (number of operational taxonomic units, evenness, phylogenetic diversity) were significantly related to hydrological factors, including distance to glacier (GD), glacier area proportion (GA), and glacier source proportion (GS), and physicochemical factors, including water temperature, pH, dissolved organic C (DOC), total N (TN), and NO3. The dominant phyla were Proteobacteria (46% of operational taxonomic units [OTUs]), Cyanobacteria (16%), Bacteroidetes (12%), Actinobacteria (9%), and Acidobacteria (6%). Microbial communities differed longitudinally along the stream at the OTU level and even at the phylum level. Correlation, canonical correlation, and network analyses showed that the microbes had significant associations with hydrological (GA, GS, and GD), biogeochemical (TN, NO3, DOC, total P, and soluble reactive P), and physicochemical (pH) factors. These results add to our knowledge of microbial community structure and potential drivers of that structure in glacier-fed stream ecosystems and provide potentially valuable data for assessing future dynamics as these systems experience further disruption caused by the influences of climate change.

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KW - Glacier-fed streams

KW - Hydrology

KW - Microbial community

KW - Microbial diversity

KW - Physicochemical factors

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U2 - 10.1086/693133

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