Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory: Simulating earth's present and past field environments

Brad M. Bebout, Steven P. Carpenter, David J. Des Marais, Mykell Discipulo, Tsegereda Embaye, Ferran Garcia-Pichel, Tori M. Hoehler, Mary Hogan, Linda L. Jahnke, Richard M. Keller, Scott R. Miller, Leslie E. Prufert-Bebout, Chris Raleigh, Michael Rothrock, Kendra Turk

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Photosynthetic microbial mat communities were obtained from marine hypersaline saltern ponds, maintained in a greenhouse facility, and examined for the effects of salinity variations. Because these microbial mats are considered to be useful analogs of ancient marine communities, they offer insights about evolutionary events during the >3 billion year time interval wherein mats co-evolved with Earth's lithosphere and atmosphere. Although photosynthetic mats can be highly dynamic and exhibit extremely high activity, the mats in the present study have been maintained for >1 year with relatively minor changes. The major groups of microorganisms, as assayed using microscopic, genetic, and biomarker methodologies, are essentially the same as those in the original field samples. Field and greenhouse mats were similar with respect to rates of exchange of oxygen and dissolved inorganic carbon across the mat-water interface, both during the day and at night. Field and greenhouse mats exhibited similar rates of efflux of methane and hydrogen. Manipulations of salinity in the water overlying the mats produced changes in the community that strongly resemble those observed in the field. A collaboratory testbed and an array of automated features are being developed to support remote scientific experimentation with the assistance of intelligent software agents. This facility will permit teams of investigators the opportunity to explore ancient environmental conditions that are rare or absent today but that might have influenced the early evolution of these photosynthetic ecosystems.

Original languageEnglish (US)
Pages (from-to)383-402
Number of pages20
JournalAstrobiology
Volume2
Issue number4
DOIs
StatePublished - Dec 1 2002

Fingerprint

microbial mat
greenhouses
Salinity
manipulators
salinity
Water
Methane
Military Personnel
dissolved inorganic carbon
Atmosphere
efflux
Ecosystem
biomarker
Hydrogen
lithosphere
Carbon
Software
pond
biomarkers
Biomarkers

Keywords

  • Biogeochemistry
  • Biomarkers
  • Microbial mat

ASJC Scopus subject areas

  • Space and Planetary Science
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory : Simulating earth's present and past field environments. / Bebout, Brad M.; Carpenter, Steven P.; Des Marais, David J.; Discipulo, Mykell; Embaye, Tsegereda; Garcia-Pichel, Ferran; Hoehler, Tori M.; Hogan, Mary; Jahnke, Linda L.; Keller, Richard M.; Miller, Scott R.; Prufert-Bebout, Leslie E.; Raleigh, Chris; Rothrock, Michael; Turk, Kendra.

In: Astrobiology, Vol. 2, No. 4, 01.12.2002, p. 383-402.

Research output: Contribution to journalArticle

Bebout, BM, Carpenter, SP, Des Marais, DJ, Discipulo, M, Embaye, T, Garcia-Pichel, F, Hoehler, TM, Hogan, M, Jahnke, LL, Keller, RM, Miller, SR, Prufert-Bebout, LE, Raleigh, C, Rothrock, M & Turk, K 2002, 'Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory: Simulating earth's present and past field environments', Astrobiology, vol. 2, no. 4, pp. 383-402. https://doi.org/10.1089/153110702762470491
Bebout, Brad M. ; Carpenter, Steven P. ; Des Marais, David J. ; Discipulo, Mykell ; Embaye, Tsegereda ; Garcia-Pichel, Ferran ; Hoehler, Tori M. ; Hogan, Mary ; Jahnke, Linda L. ; Keller, Richard M. ; Miller, Scott R. ; Prufert-Bebout, Leslie E. ; Raleigh, Chris ; Rothrock, Michael ; Turk, Kendra. / Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory : Simulating earth's present and past field environments. In: Astrobiology. 2002 ; Vol. 2, No. 4. pp. 383-402.
@article{4d83a736fcce4116addba8aeb36657e2,
title = "Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory: Simulating earth's present and past field environments",
abstract = "Photosynthetic microbial mat communities were obtained from marine hypersaline saltern ponds, maintained in a greenhouse facility, and examined for the effects of salinity variations. Because these microbial mats are considered to be useful analogs of ancient marine communities, they offer insights about evolutionary events during the >3 billion year time interval wherein mats co-evolved with Earth's lithosphere and atmosphere. Although photosynthetic mats can be highly dynamic and exhibit extremely high activity, the mats in the present study have been maintained for >1 year with relatively minor changes. The major groups of microorganisms, as assayed using microscopic, genetic, and biomarker methodologies, are essentially the same as those in the original field samples. Field and greenhouse mats were similar with respect to rates of exchange of oxygen and dissolved inorganic carbon across the mat-water interface, both during the day and at night. Field and greenhouse mats exhibited similar rates of efflux of methane and hydrogen. Manipulations of salinity in the water overlying the mats produced changes in the community that strongly resemble those observed in the field. A collaboratory testbed and an array of automated features are being developed to support remote scientific experimentation with the assistance of intelligent software agents. This facility will permit teams of investigators the opportunity to explore ancient environmental conditions that are rare or absent today but that might have influenced the early evolution of these photosynthetic ecosystems.",
keywords = "Biogeochemistry, Biomarkers, Microbial mat",
author = "Bebout, {Brad M.} and Carpenter, {Steven P.} and {Des Marais}, {David J.} and Mykell Discipulo and Tsegereda Embaye and Ferran Garcia-Pichel and Hoehler, {Tori M.} and Mary Hogan and Jahnke, {Linda L.} and Keller, {Richard M.} and Miller, {Scott R.} and Prufert-Bebout, {Leslie E.} and Chris Raleigh and Michael Rothrock and Kendra Turk",
year = "2002",
month = "12",
day = "1",
doi = "10.1089/153110702762470491",
language = "English (US)",
volume = "2",
pages = "383--402",
journal = "Astrobiology",
issn = "1531-1074",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory

T2 - Simulating earth's present and past field environments

AU - Bebout, Brad M.

AU - Carpenter, Steven P.

AU - Des Marais, David J.

AU - Discipulo, Mykell

AU - Embaye, Tsegereda

AU - Garcia-Pichel, Ferran

AU - Hoehler, Tori M.

AU - Hogan, Mary

AU - Jahnke, Linda L.

AU - Keller, Richard M.

AU - Miller, Scott R.

AU - Prufert-Bebout, Leslie E.

AU - Raleigh, Chris

AU - Rothrock, Michael

AU - Turk, Kendra

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Photosynthetic microbial mat communities were obtained from marine hypersaline saltern ponds, maintained in a greenhouse facility, and examined for the effects of salinity variations. Because these microbial mats are considered to be useful analogs of ancient marine communities, they offer insights about evolutionary events during the >3 billion year time interval wherein mats co-evolved with Earth's lithosphere and atmosphere. Although photosynthetic mats can be highly dynamic and exhibit extremely high activity, the mats in the present study have been maintained for >1 year with relatively minor changes. The major groups of microorganisms, as assayed using microscopic, genetic, and biomarker methodologies, are essentially the same as those in the original field samples. Field and greenhouse mats were similar with respect to rates of exchange of oxygen and dissolved inorganic carbon across the mat-water interface, both during the day and at night. Field and greenhouse mats exhibited similar rates of efflux of methane and hydrogen. Manipulations of salinity in the water overlying the mats produced changes in the community that strongly resemble those observed in the field. A collaboratory testbed and an array of automated features are being developed to support remote scientific experimentation with the assistance of intelligent software agents. This facility will permit teams of investigators the opportunity to explore ancient environmental conditions that are rare or absent today but that might have influenced the early evolution of these photosynthetic ecosystems.

AB - Photosynthetic microbial mat communities were obtained from marine hypersaline saltern ponds, maintained in a greenhouse facility, and examined for the effects of salinity variations. Because these microbial mats are considered to be useful analogs of ancient marine communities, they offer insights about evolutionary events during the >3 billion year time interval wherein mats co-evolved with Earth's lithosphere and atmosphere. Although photosynthetic mats can be highly dynamic and exhibit extremely high activity, the mats in the present study have been maintained for >1 year with relatively minor changes. The major groups of microorganisms, as assayed using microscopic, genetic, and biomarker methodologies, are essentially the same as those in the original field samples. Field and greenhouse mats were similar with respect to rates of exchange of oxygen and dissolved inorganic carbon across the mat-water interface, both during the day and at night. Field and greenhouse mats exhibited similar rates of efflux of methane and hydrogen. Manipulations of salinity in the water overlying the mats produced changes in the community that strongly resemble those observed in the field. A collaboratory testbed and an array of automated features are being developed to support remote scientific experimentation with the assistance of intelligent software agents. This facility will permit teams of investigators the opportunity to explore ancient environmental conditions that are rare or absent today but that might have influenced the early evolution of these photosynthetic ecosystems.

KW - Biogeochemistry

KW - Biomarkers

KW - Microbial mat

UR - http://www.scopus.com/inward/record.url?scp=0013275364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0013275364&partnerID=8YFLogxK

U2 - 10.1089/153110702762470491

DO - 10.1089/153110702762470491

M3 - Article

C2 - 12593778

AN - SCOPUS:0013275364

VL - 2

SP - 383

EP - 402

JO - Astrobiology

JF - Astrobiology

SN - 1531-1074

IS - 4

ER -