TY - JOUR
T1 - Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces
AU - Brafman, David A.
AU - Chang, Chien W.
AU - Fernandez, Antonio
AU - Willert, Karl
AU - Varghese, Shyni
AU - Chien, Shu
N1 - Funding Information:
D.A.B. was supported by funding from the University of California Biotechnology Research and Education Program ( 2007-006 ), the National Science Foundation Graduate Research Fellowship Program , and the UCSD California Institute of Regenerative Postdoctoral Fellowship Program . This research was supported in part by the California Institute of Regenerative Medicine ( RS1-00172-1 ) to S.C., and NHLBI Research Grant HL080518 to S.C. We would also like to thank the UCSD Human Embryonic Core Facility for generation and characterization of the hiPSC lines.
PY - 2010/12
Y1 - 2010/12
N2 - Realization of the full potential of human pluripotent stem cells (hPSCs) in regenerative medicine requires the development of well-defined culture conditions for their long-term growth and directed differentiation. Current practices for maintaining hPSCs generally utilize empirically determined combinations of feeder cells and other animal-based products, which are expensive, difficult to isolate, subject to batch-to-batch variations, and unsuitable for cell-based therapies. Using a high-throughput screening approach, we identified several polymers that can support self-renewal of hPSCs. While most of these polymers provide support for only a short period of time, we identified a synthetic polymer poly(methyl vinyl ether-alt-maleic anhydride) (PMVE-alt-MA) that supported the long-term attachment, proliferation and self-renewal of HUES1, HUES9, and iPSCs. The hPSCs cultured on PMVE-alt-MA maintained their characteristic morphology, expressed high levels of markers of pluripotency, and retained a normal karyotype. Such cost-effective, polymer-based matrices that support long-term self-renewal and proliferation of hPSCs will not only help to accelerate the translational perspectives of hPSCs, but also provide a platform to elucidate the underlying molecular mechanisms that regulate stem cell proliferation and differentiation.
AB - Realization of the full potential of human pluripotent stem cells (hPSCs) in regenerative medicine requires the development of well-defined culture conditions for their long-term growth and directed differentiation. Current practices for maintaining hPSCs generally utilize empirically determined combinations of feeder cells and other animal-based products, which are expensive, difficult to isolate, subject to batch-to-batch variations, and unsuitable for cell-based therapies. Using a high-throughput screening approach, we identified several polymers that can support self-renewal of hPSCs. While most of these polymers provide support for only a short period of time, we identified a synthetic polymer poly(methyl vinyl ether-alt-maleic anhydride) (PMVE-alt-MA) that supported the long-term attachment, proliferation and self-renewal of HUES1, HUES9, and iPSCs. The hPSCs cultured on PMVE-alt-MA maintained their characteristic morphology, expressed high levels of markers of pluripotency, and retained a normal karyotype. Such cost-effective, polymer-based matrices that support long-term self-renewal and proliferation of hPSCs will not only help to accelerate the translational perspectives of hPSCs, but also provide a platform to elucidate the underlying molecular mechanisms that regulate stem cell proliferation and differentiation.
KW - Defined culture conditions
KW - Extracellular matrix
KW - Human embryonic stem cells
KW - Human induced pluripotent stem cells
KW - Polymer arrays
UR - http://www.scopus.com/inward/record.url?scp=77957298109&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957298109&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2010.08.007
DO - 10.1016/j.biomaterials.2010.08.007
M3 - Article
C2 - 20817292
AN - SCOPUS:77957298109
SN - 0142-9612
VL - 31
SP - 9135
EP - 9144
JO - Biomaterials
JF - Biomaterials
IS - 34
ER -