Localization of the steroid 1‐dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy

B. Wagner, P. G. Atrat, J. E. Clark‐Curtiss, M. Wagner

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The steroid 1‐dehydrogenase of Rhodococcus erythropolis IMET 7030, an active steroid‐transforming strain, was localized by immunogold labelling both in cells induced with 17‐alpha‐methyl‐testosterone and in noninduced cells. The labelling intensity was much higher in induced cells than in noninduced cells, indicating increased enzyme production in the case of induction. Using the postembedding procedure, the main portion of the enzyme was found in the peripheral region of the cytoplasm. A considerable amount was bound in clusters to the inner side of the cytoplasmic membrane. The enzyme was also detected in channels connecting the cytoplasm with the cell surface. By means of the preembedding labelling, a few gold clusters could be detected on the cell surface. The significance of this observation was discussed.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of Basic Microbiology
Volume32
Issue number1
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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