Localization of the steroid 1-dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy.

B. Wagner, P. G. Atrat, J. E. Clark-Curtiss, M. Wagner

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The steroid 1-dehydrogenase of Rhodococcus erythropolis IMET 7030, an active steroid-transforming strain, was localized by immunogold labelling both in cells induced with 17-alpha-methyl-testosterone and in noninduced cells. The labelling intensity was much higher in induced cells than in noninduced cells, indicating increased enzyme production in the case of induction. Using the postembedding procedure, the main portion of the enzyme was found in the peripheral region of the cytoplasm. A considerable amount was bound in clusters to the inner side of the cytoplasmic membrane. The enzyme was also detected in channels connecting the cytoplasm with the cell surface. By means of the preembedding labelling, a few gold clusters could be detected on the cell surface. The significance of this observation was discussed.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalJournal of Basic Microbiology
Volume32
Issue number1
StatePublished - 1992
Externally publishedYes

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3-oxosteroid delta(1) dehydrogenase
Rhodococcus
Immunoelectron Microscopy
Cytoplasm
Epitestosterone
Enzymes
Gold
histaminol
Steroids
Cell Membrane

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Localization of the steroid 1-dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy. / Wagner, B.; Atrat, P. G.; Clark-Curtiss, J. E.; Wagner, M.

In: Journal of Basic Microbiology, Vol. 32, No. 1, 1992, p. 65-71.

Research output: Contribution to journalArticle

Wagner, B. ; Atrat, P. G. ; Clark-Curtiss, J. E. ; Wagner, M. / Localization of the steroid 1-dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy. In: Journal of Basic Microbiology. 1992 ; Vol. 32, No. 1. pp. 65-71.
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