TY - JOUR
T1 - Localisation and accumulation of a new carotenoporphyrin in two primary tumour models
AU - Van Den Akker, J. T H M
AU - Speelman, O. C.
AU - Van Staveren, H. J.
AU - Moore, Ana
AU - Moore, Thomas
AU - Gust, D.
AU - Star, W. M.
AU - Sterenborg, H. J C M
N1 - Funding Information:
This work was supported by the European Community, contract number BMH4-CT97-2260 (J.T.H.M.v.d.A.) and the Dutch Cancer Society, project number DDHK-95-1062 (O.C.S. and H.J.v.S.). The authors wish to thank Konnie Hebeda for her assistance in analysing the haematoxylin–eosin-stained sections and Max Witjes for performing the EAI grading.
PY - 2000/2/29
Y1 - 2000/2/29
N2 - We have investigated the tumour-localising properties and in vivo fluorescence kinetics of a hexamethoxylated carotenoporphyrin (CP6) in two primary tumour models: UV-B-induced early skin cancer in hairless mice and chemically induced mucosal dysplasia in the rat palate. CP6 fluorescence kinetics are investigated by measuring in vivo fluorescence spectra and images of the mouse skin and the rat palate at different time points after injection. For the tumour-localising properties, microscopic phase-contrast and fluorescence images are recorded. The in vivo fluorescence kinetics in the mouse skin show localization of CP6 in the tumours. However, fluorescence microscopy images show that CP6 localises in the dermis and structures that are not related to the malignant transformation of the mouse skin. The fluorescence kinetics in the rat palate show a significant correlation between the degree of malignancy and the CP6 fluorescence build-up time in the palate. The microscopic images show that CP6 fluorescence localises in the connective tissue and not in the dysplastic epithelium. In conclusion, CP6 does not localise preferentially in (pre-) cancerous tissue in the two primary tumour models studied here, in contrast to reports about localisation of carotenoporphyrins in transplanted tumours. However, the CP6 build-up time in rat palates correlates with the degree of malignancy and this might possibly be a useful parameter in tumour detection. (C) 2000 Elsevier Science S.A. All rights reserved.
AB - We have investigated the tumour-localising properties and in vivo fluorescence kinetics of a hexamethoxylated carotenoporphyrin (CP6) in two primary tumour models: UV-B-induced early skin cancer in hairless mice and chemically induced mucosal dysplasia in the rat palate. CP6 fluorescence kinetics are investigated by measuring in vivo fluorescence spectra and images of the mouse skin and the rat palate at different time points after injection. For the tumour-localising properties, microscopic phase-contrast and fluorescence images are recorded. The in vivo fluorescence kinetics in the mouse skin show localization of CP6 in the tumours. However, fluorescence microscopy images show that CP6 localises in the dermis and structures that are not related to the malignant transformation of the mouse skin. The fluorescence kinetics in the rat palate show a significant correlation between the degree of malignancy and the CP6 fluorescence build-up time in the palate. The microscopic images show that CP6 fluorescence localises in the connective tissue and not in the dysplastic epithelium. In conclusion, CP6 does not localise preferentially in (pre-) cancerous tissue in the two primary tumour models studied here, in contrast to reports about localisation of carotenoporphyrins in transplanted tumours. However, the CP6 build-up time in rat palates correlates with the degree of malignancy and this might possibly be a useful parameter in tumour detection. (C) 2000 Elsevier Science S.A. All rights reserved.
KW - Carotenoporphyrin
KW - Fluorescence microscopy
KW - Fluorescence spectroscopy
KW - In vivo fluorescence kinetics
KW - Tumour detection
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U2 - 10.1016/S1011-1344(99)00157-8
DO - 10.1016/S1011-1344(99)00157-8
M3 - Article
C2 - 10836539
AN - SCOPUS:0034101720
SN - 1011-1344
VL - 54
SP - 108
EP - 115
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
IS - 2-3
ER -