Local electrical potential detection of DNA by nanowire-nanopore sensors

Ping Xie, Qihua Xiong, Ying Fang, Quan Qing, Charles M. Lieber

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

Nanopores could potentially be used to perform single-molecule DNA sequencing at low cost and with high throughput. Although single base resolution and differentiation have been demonstrated with nanopores using ionic current measurements, direct sequencing has not been achieved because of the difficulties in recording very small (∼pA) ionic currents at a bandwidth consistent with fast translocation speeds. Here, we show that solid-state nanopores can be combined with silicon nanowire field-effect transistors to create sensors in which detection is localized and self-aligned at the nanopore. Well-defined field-effect transistor signals associated with DNA translocation are recorded when an ionic strength gradient is imposed across the nanopores. Measurements and modelling show that field-effect transistor signals are generated by highly localized changes in the electrical potential during DNA translocation, and that nanowire-nanopore sensors could enable large-scale integration with a high intrinsic bandwidth.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalNature Nanotechnology
Volume7
Issue number2
DOIs
StatePublished - Feb 2012
Externally publishedYes

Fingerprint

Nanopores
Nanowires
DNA
nanowires
sequencing
field effect transistors
deoxyribonucleic acid
sensors
Sensors
Field effect transistors
bandwidth
large scale integration
recording
solid state
Bandwidth
LSI circuits
gradients
silicon
Electric current measurement
Silicon

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics

Cite this

Local electrical potential detection of DNA by nanowire-nanopore sensors. / Xie, Ping; Xiong, Qihua; Fang, Ying; Qing, Quan; Lieber, Charles M.

In: Nature Nanotechnology, Vol. 7, No. 2, 02.2012, p. 119-125.

Research output: Contribution to journalArticle

Xie, Ping ; Xiong, Qihua ; Fang, Ying ; Qing, Quan ; Lieber, Charles M. / Local electrical potential detection of DNA by nanowire-nanopore sensors. In: Nature Nanotechnology. 2012 ; Vol. 7, No. 2. pp. 119-125.
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