Load-dependent detachment kinetics plays a key role in bidirectional cargo transport by kinesin and dynein

Kazuka G. Ohashi, Lifeng Han, Brandon Mentley, Jiaxuan Wang, John Fricks, William O. Hancock

Research output: Contribution to journalArticle

Abstract

Bidirectional cargo transport along microtubules is carried out by opposing teams of kinesin and dynein motors. Despite considerable study, the factors that determine whether these competing teams achieve net anterograde or retrograde transport in cells remain unclear. The goal of this work is to use stochastic simulations of bidirectional transport to determine the motor properties that most strongly determine overall cargo velocity and directionality. Simulations were carried out based on published optical tweezer characterization of kinesin-1 and kinesin-2, and for available data for cytoplasmic dynein and the dynein-dynactin-BicD2 (DDB) complex. By varying dynein parameters and analyzing cargo trajectories, we find that net cargo transport is predicted to depend minimally on the dynein stall force, but strongly on dynein load-dependent detachment kinetics. In simulations, dynein is dominated by kinesin-1, but DDB and kinesin-1 are evenly matched, recapitulating recent experimental work. Kinesin-2 competes less well against dynein and DDB, and overall, load-dependent motor detachment is the property that most determines a motor's ability to compete in bidirectional transport. It follows that the most effective intracellular regulators of bidirectional transport are predicted to be those that alter motor detachment kinetics rather than motor velocity or stall force.

Original languageEnglish (US)
Pages (from-to)284-294
Number of pages11
JournalTraffic
Volume20
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Dyneins
Kinesin
Kinetics
Cytoplasmic Dyneins
Optical Tweezers
Optical tweezers
Microtubules
Trajectories

Keywords

  • axon
  • dynein
  • Kinesin
  • microtubule
  • motor protein
  • neuron
  • optical tweezer
  • single-molecule
  • transport
  • vesicle

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Load-dependent detachment kinetics plays a key role in bidirectional cargo transport by kinesin and dynein. / Ohashi, Kazuka G.; Han, Lifeng; Mentley, Brandon; Wang, Jiaxuan; Fricks, John; Hancock, William O.

In: Traffic, Vol. 20, No. 4, 01.04.2019, p. 284-294.

Research output: Contribution to journalArticle

Ohashi, Kazuka G. ; Han, Lifeng ; Mentley, Brandon ; Wang, Jiaxuan ; Fricks, John ; Hancock, William O. / Load-dependent detachment kinetics plays a key role in bidirectional cargo transport by kinesin and dynein. In: Traffic. 2019 ; Vol. 20, No. 4. pp. 284-294.
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