Live single cell rotation using hydrodynamic microvortex flow and optical trapping

R. M. Shetty, J. R. Myers, W. L. Teller, A. Shabilla, D. Smith, J. Houkal, J. Vela, Shih-Hui Chao, R. H. Johnson, L. Kelbauskas, H. Wang, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a method to stably rotate cells about a rotation axis perpendicular to the imaging axis, using a combination of hydrodynamic microvortex flow and optical trapping on a microfluidic chip. We present theoretical simulations, fabrication, system setup, rotation imaging, and data analysis. A tilted backside exposure technique was used to fabricate trapezoidal features which form the site for microvortical flow. Optical trapping was used to hold the cell in this flow, thereby stabilizing cell rotation. The integrated setup can be utilized for 3D single cell imaging with sub-micron isotropic spatial resolution.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1545-1547
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Fluorescence imaging
  • Microvortex
  • Optical tweezers
  • Single cell rotation

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Shetty, R. M., Myers, J. R., Teller, W. L., Shabilla, A., Smith, D., Houkal, J., Vela, J., Chao, S-H., Johnson, R. H., Kelbauskas, L., Wang, H., & Meldrum, D. (2014). Live single cell rotation using hydrodynamic microvortex flow and optical trapping. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1545-1547). Chemical and Biological Microsystems Society.