Live single cell rotation using hydrodynamic microvortex flow and optical trapping

R. M. Shetty; J. R. Myers; W. L. Teller; A. Shabilla; D. Smith; J. Houkal; J. Vela; Shih-Hui Chao; R. H. Johnson; L. Kelbauskas; H. Wang; Deirdre Meldrum

Live single cell rotation using hydrodynamic microvortex flow and optical trapping

R. M. Shetty, J. R. Myers, W. L. Teller, A. Shabilla, D. Smith, J. Houkal, J. Vela, Shih-Hui Chao, R. H. Johnson, L. Kelbauskas, H. Wang, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present a method to stably rotate cells about a rotation axis perpendicular to the imaging axis, using a combination of hydrodynamic microvortex flow and optical trapping on a microfluidic chip. We present theoretical simulations, fabrication, system setup, rotation imaging, and data analysis. A tilted backside exposure technique was used to fabricate trapezoidal features which form the site for microvortical flow. Optical trapping was used to hold the cell in this flow, thereby stabilizing cell rotation. The integrated setup can be utilized for 3D single cell imaging with sub-micron isotropic spatial resolution.

Original language	English (US)
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	1545-1547
Number of pages	3
ISBN (Print)	9780979806476
State	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	10/26/14 → 10/30/14

Keywords

Fluorescence imaging
Microvortex
Optical tweezers
Single cell rotation

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Shetty, R. M., Myers, J. R., Teller, W. L., Shabilla, A., Smith, D., Houkal, J., Vela, J., Chao, S.-H., Johnson, R. H., Kelbauskas, L., Wang, H., & Meldrum, D. (2014). Live single cell rotation using hydrodynamic microvortex flow and optical trapping. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1545-1547). Chemical and Biological Microsystems Society.

Live single cell rotation using hydrodynamic microvortex flow and optical trapping. / Shetty, R. M.; Myers, J. R.; Teller, W. L. et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1545-1547.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shetty, RM, Myers, JR, Teller, WL, Shabilla, A, Smith, D, Houkal, J, Vela, J, Chao, S-H, Johnson, RH, Kelbauskas, L, Wang, H & Meldrum, D 2014, Live single cell rotation using hydrodynamic microvortex flow and optical trapping. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 1545-1547, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.

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title = "Live single cell rotation using hydrodynamic microvortex flow and optical trapping",

abstract = "We present a method to stably rotate cells about a rotation axis perpendicular to the imaging axis, using a combination of hydrodynamic microvortex flow and optical trapping on a microfluidic chip. We present theoretical simulations, fabrication, system setup, rotation imaging, and data analysis. A tilted backside exposure technique was used to fabricate trapezoidal features which form the site for microvortical flow. Optical trapping was used to hold the cell in this flow, thereby stabilizing cell rotation. The integrated setup can be utilized for 3D single cell imaging with sub-micron isotropic spatial resolution.",

keywords = "Fluorescence imaging, Microvortex, Optical tweezers, Single cell rotation",

author = "Shetty, {R. M.} and Myers, {J. R.} and Teller, {W. L.} and A. Shabilla and D. Smith and J. Houkal and J. Vela and Shih-Hui Chao and Johnson, {R. H.} and L. Kelbauskas and H. Wang and Deirdre Meldrum",

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booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

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note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

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TY - GEN

T1 - Live single cell rotation using hydrodynamic microvortex flow and optical trapping

AU - Shetty, R. M.

AU - Myers, J. R.

AU - Teller, W. L.

AU - Shabilla, A.

AU - Smith, D.

AU - Houkal, J.

AU - Vela, J.

AU - Chao, Shih-Hui

AU - Johnson, R. H.

AU - Kelbauskas, L.

AU - Wang, H.

AU - Meldrum, Deirdre

PY - 2014

Y1 - 2014

N2 - We present a method to stably rotate cells about a rotation axis perpendicular to the imaging axis, using a combination of hydrodynamic microvortex flow and optical trapping on a microfluidic chip. We present theoretical simulations, fabrication, system setup, rotation imaging, and data analysis. A tilted backside exposure technique was used to fabricate trapezoidal features which form the site for microvortical flow. Optical trapping was used to hold the cell in this flow, thereby stabilizing cell rotation. The integrated setup can be utilized for 3D single cell imaging with sub-micron isotropic spatial resolution.

AB - We present a method to stably rotate cells about a rotation axis perpendicular to the imaging axis, using a combination of hydrodynamic microvortex flow and optical trapping on a microfluidic chip. We present theoretical simulations, fabrication, system setup, rotation imaging, and data analysis. A tilted backside exposure technique was used to fabricate trapezoidal features which form the site for microvortical flow. Optical trapping was used to hold the cell in this flow, thereby stabilizing cell rotation. The integrated setup can be utilized for 3D single cell imaging with sub-micron isotropic spatial resolution.

KW - Fluorescence imaging

KW - Microvortex

KW - Optical tweezers

KW - Single cell rotation

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M3 - Conference contribution

AN - SCOPUS:84941670170

SN - 9780979806476

SP - 1545

EP - 1547

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

Live single cell rotation using hydrodynamic microvortex flow and optical trapping

Abstract

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Keywords

ASJC Scopus subject areas

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