Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays

Rohit Mishra, Rizwan Alam, David J. Kinahan, Karen Anderson, Jens Ducree

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

We demonstrate centrifugal [liquid handling] automation of an Enzyme-Linked Immuno-Sorbent Assay (ELISA) for the detection of anti-p53 antibodies in whole blood. On this 'Lab-on-a-Disc' (LoaD) platform, all unit operations were implemented by event-triggered rotational flow control. In order to avoid interference during absorbance measurement from the solid phase in this heterogeneous assay format, it is pivotal that the intermediate reaction product is eventually forwarded from the incubation chamber to a distinct optical measurement chamber. To this end we have devised routing of flows by lipophilic film valves (LFVs) which remain intact in aqueous and selectively dissolve when exposed to an ancillary, oleophilic solvent.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages523-526
Number of pages4
Volume2015-February
EditionFebruary
DOIs
StatePublished - Feb 26 2015
Event2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, Portugal
Duration: Jan 18 2015Jan 22 2015

Other

Other2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015
CountryPortugal
CityEstoril
Period1/18/151/22/15

Fingerprint

immunoassay
automation
Assays
Automation
chambers
rotational flow
Rotational flow
membranes
Membranes
sorbents
Sorbents
antibodies
Reaction products
optical measurement
Flow control
Antibodies
reaction products
blood
format
solid phases

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Mishra, R., Alam, R., Kinahan, D. J., Anderson, K., & Ducree, J. (2015). Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (February ed., Vol. 2015-February, pp. 523-526). [7051007] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2015.7051007

Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. / Mishra, Rohit; Alam, Rizwan; Kinahan, David J.; Anderson, Karen; Ducree, Jens.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). Vol. 2015-February February. ed. Institute of Electrical and Electronics Engineers Inc., 2015. p. 523-526 7051007.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mishra, R, Alam, R, Kinahan, DJ, Anderson, K & Ducree, J 2015, Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). February edn, vol. 2015-February, 7051007, Institute of Electrical and Electronics Engineers Inc., pp. 523-526, 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015, Estoril, Portugal, 1/18/15. https://doi.org/10.1109/MEMSYS.2015.7051007
Mishra R, Alam R, Kinahan DJ, Anderson K, Ducree J. Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). February ed. Vol. 2015-February. Institute of Electrical and Electronics Engineers Inc. 2015. p. 523-526. 7051007 https://doi.org/10.1109/MEMSYS.2015.7051007
Mishra, Rohit ; Alam, Rizwan ; Kinahan, David J. ; Anderson, Karen ; Ducree, Jens. / Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). Vol. 2015-February February. ed. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 523-526
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