Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays

Rohit Mishra; Rizwan Alam; David J. Kinahan; Karen Anderson; Jens Ducree

doi:10.1109/MEMSYS.2015.7051007

Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays

Rohit Mishra, Rizwan Alam, David J. Kinahan, Karen Anderson, Jens Ducree

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

9 Scopus citations

Abstract

We demonstrate centrifugal [liquid handling] automation of an Enzyme-Linked Immuno-Sorbent Assay (ELISA) for the detection of anti-p53 antibodies in whole blood. On this 'Lab-on-a-Disc' (LoaD) platform, all unit operations were implemented by event-triggered rotational flow control. In order to avoid interference during absorbance measurement from the solid phase in this heterogeneous assay format, it is pivotal that the intermediate reaction product is eventually forwarded from the incubation chamber to a distinct optical measurement chamber. To this end we have devised routing of flows by lipophilic film valves (LFVs) which remain intact in aqueous and selectively dissolve when exposed to an ancillary, oleophilic solvent.

Original language	English (US)
Title of host publication	2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	523-526
Number of pages	4
Edition	February
ISBN (Electronic)	9781479979554
DOIs	https://doi.org/10.1109/MEMSYS.2015.7051007
State	Published - Feb 26 2015
Event	2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, Portugal Duration: Jan 18 2015 → Jan 22 2015

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Number	February
Volume	2015-February
ISSN (Print)	1084-6999

Other

Other	2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015
Country/Territory	Portugal
City	Estoril
Period	1/18/15 → 1/22/15

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2015.7051007

Cite this

Mishra, R., Alam, R., Kinahan, D. J., Anderson, K., & Ducree, J. (2015). Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. In 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 (February ed., pp. 523-526). Article 7051007 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2015-February, No. February). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2015.7051007

Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. / Mishra, Rohit; Alam, Rizwan; Kinahan, David J. et al.
2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February. ed. Institute of Electrical and Electronics Engineers Inc., 2015. p. 523-526 7051007 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2015-February, No. February).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Mishra, R, Alam, R, Kinahan, DJ, Anderson, K & Ducree, J 2015, Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. in 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February edn, 7051007, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), no. February, vol. 2015-February, Institute of Electrical and Electronics Engineers Inc., pp. 523-526, 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015, Estoril, Portugal, 1/18/15. https://doi.org/10.1109/MEMSYS.2015.7051007

Mishra R, Alam R, Kinahan DJ, Anderson K, Ducree J. Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. In 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February ed. Institute of Electrical and Electronics Engineers Inc. 2015. p. 523-526. 7051007. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); February). doi: 10.1109/MEMSYS.2015.7051007

Mishra, Rohit ; Alam, Rizwan ; Kinahan, David J. et al. / Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays. 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February. ed. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 523-526 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); February).

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abstract = "We demonstrate centrifugal [liquid handling] automation of an Enzyme-Linked Immuno-Sorbent Assay (ELISA) for the detection of anti-p53 antibodies in whole blood. On this 'Lab-on-a-Disc' (LoaD) platform, all unit operations were implemented by event-triggered rotational flow control. In order to avoid interference during absorbance measurement from the solid phase in this heterogeneous assay format, it is pivotal that the intermediate reaction product is eventually forwarded from the incubation chamber to a distinct optical measurement chamber. To this end we have devised routing of flows by lipophilic film valves (LFVs) which remain intact in aqueous and selectively dissolve when exposed to an ancillary, oleophilic solvent.",

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AB - We demonstrate centrifugal [liquid handling] automation of an Enzyme-Linked Immuno-Sorbent Assay (ELISA) for the detection of anti-p53 antibodies in whole blood. On this 'Lab-on-a-Disc' (LoaD) platform, all unit operations were implemented by event-triggered rotational flow control. In order to avoid interference during absorbance measurement from the solid phase in this heterogeneous assay format, it is pivotal that the intermediate reaction product is eventually forwarded from the incubation chamber to a distinct optical measurement chamber. To this end we have devised routing of flows by lipophilic film valves (LFVs) which remain intact in aqueous and selectively dissolve when exposed to an ancillary, oleophilic solvent.

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Lipophilic-membrane based routing for centrifugal automation of heterogeneous immunoassays

Abstract

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