Lipidic phase membrane protein serial femtosecond crystallography

Linda C. Johansson; David Arnlund; Thomas A. White; Gergely Katona; Daniel P. Deponte; Uwe Weierstall; R. Bruce Doak; Robert L. Shoeman; Lukas Lomb; Erik Malmerberg; Jan Davidsson; Karol Nass; Mengning Liang; Jakob Andreasson; Andrew Aquila; Sasa Bajt; Miriam Barthelmess; Anton Barty; Michael J. Bogan; Christoph Bostedt; John D. Bozek; Carl Caleman; Ryan Coffee; Nicola Coppola; Tomas Ekeberg; Sascha W. Epp; Benjamin Erk; Holger Fleckenstein; Lutz Foucar; Heinz Graafsma; Lars Gumprecht; Janos Hajdu; Christina Y. Hampton; Robert Hartmann; Andreas Hartmann; Gülnter Hauser; Helmut Hirsemann; Peter Holl; Mark S. Hunter; Stephan Kassemeyer; Nils Kimmel; Richard Kirian; Filipe R N C Maia; Stefano Marchesini; Andrew V. Martin; Christian Reich; Daniel Rolles; Benedikt Rudek; Artem Rudenko; Ilme Schlichting; Joachim Schulz; M. Marvin Seibert; Raymond G. Sierra; Heike Soltau; Dmitri Starodub; Francesco Stellato; Stephan Stern; Lothar Strülder; Nicusor Timneanu; Joachim Ullrich; Weixiao Y. Wahlgren; Xiaoyu Wang; Georg Weidenspointner; Cornelia Wunderer; Petra Fromme; Henry N. Chapman; John Spence; Richard Neutze

doi:10.1038/nmeth.1867

Lipidic phase membrane protein serial femtosecond crystallography

Linda C. Johansson, David Arnlund, Thomas A. White, Gergely Katona, Daniel P. Deponte, Uwe Weierstall, R. Bruce Doak, Robert L. Shoeman, Lukas Lomb, Erik Malmerberg, Jan Davidsson, Karol Nass, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph BostedtJohn D. Bozek, Carl Caleman, Ryan Coffee, Nicola Coppola, Tomas Ekeberg, Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Robert Hartmann, Andreas Hartmann, Gülnter Hauser, Helmut Hirsemann, Peter Holl, Mark S. Hunter, Stephan Kassemeyer, Nils Kimmel, Richard Kirian, Filipe R N C Maia, Stefano Marchesini, Andrew V. Martin, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M. Marvin Seibert, Raymond G. Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strülder, Nicusor Timneanu, Joachim Ullrich, Weixiao Y. Wahlgren, Xiaoyu Wang, Georg Weidenspointner, Cornelia Wunderer, Petra Fromme, Henry N. Chapman, John Spence, Richard Neutze

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Abstract

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Original language	English (US)
Pages (from-to)	263-265
Number of pages	3
Journal	Nature Methods
Volume	9
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.1867
State	Published - Mar 2012

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Johansson, L. C., Arnlund, D., White, T. A., Katona, G., Deponte, D. P., Weierstall, U., Doak, R. B., Shoeman, R. L., Lomb, L., Malmerberg, E., Davidsson, J., Nass, K., Liang, M., Andreasson, J., Aquila, A., Bajt, S., Barthelmess, M., Barty, A., Bogan, M. J., ... Neutze, R. (2012). Lipidic phase membrane protein serial femtosecond crystallography. Nature Methods, 9(3), 263-265. https://doi.org/10.1038/nmeth.1867

Johansson, LC, Arnlund, D, White, TA, Katona, G, Deponte, DP, Weierstall, U, Doak, RB, Shoeman, RL, Lomb, L, Malmerberg, E, Davidsson, J, Nass, K, Liang, M, Andreasson, J, Aquila, A, Bajt, S, Barthelmess, M, Barty, A, Bogan, MJ, Bostedt, C, Bozek, JD, Caleman, C, Coffee, R, Coppola, N, Ekeberg, T, Epp, SW, Erk, B, Fleckenstein, H, Foucar, L, Graafsma, H, Gumprecht, L, Hajdu, J, Hampton, CY, Hartmann, R, Hartmann, A, Hauser, G, Hirsemann, H, Holl, P, Hunter, MS, Kassemeyer, S, Kimmel, N, Kirian, R, Maia, FRNC, Marchesini, S, Martin, AV, Reich, C, Rolles, D, Rudek, B, Rudenko, A, Schlichting, I, Schulz, J, Seibert, MM, Sierra, RG, Soltau, H, Starodub, D, Stellato, F, Stern, S, Strülder, L, Timneanu, N, Ullrich, J, Wahlgren, WY, Wang, X, Weidenspointner, G, Wunderer, C, Fromme, P, Chapman, HN, Spence, J & Neutze, R 2012, 'Lipidic phase membrane protein serial femtosecond crystallography', Nature Methods, vol. 9, no. 3, pp. 263-265. https://doi.org/10.1038/nmeth.1867

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title = "Lipidic phase membrane protein serial femtosecond crystallography",

abstract = "X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.",

author = "Johansson, {Linda C.} and David Arnlund and White, {Thomas A.} and Gergely Katona and Deponte, {Daniel P.} and Uwe Weierstall and Doak, {R. Bruce} and Shoeman, {Robert L.} and Lukas Lomb and Erik Malmerberg and Jan Davidsson and Karol Nass and Mengning Liang and Jakob Andreasson and Andrew Aquila and Sasa Bajt and Miriam Barthelmess and Anton Barty and Bogan, {Michael J.} and Christoph Bostedt and Bozek, {John D.} and Carl Caleman and Ryan Coffee and Nicola Coppola and Tomas Ekeberg and Epp, {Sascha W.} and Benjamin Erk and Holger Fleckenstein and Lutz Foucar and Heinz Graafsma and Lars Gumprecht and Janos Hajdu and Hampton, {Christina Y.} and Robert Hartmann and Andreas Hartmann and G{\"u}lnter Hauser and Helmut Hirsemann and Peter Holl and Hunter, {Mark S.} and Stephan Kassemeyer and Nils Kimmel and Richard Kirian and Maia, {Filipe R N C} and Stefano Marchesini and Martin, {Andrew V.} and Christian Reich and Daniel Rolles and Benedikt Rudek and Artem Rudenko and Ilme Schlichting and Joachim Schulz and Seibert, {M. Marvin} and Sierra, {Raymond G.} and Heike Soltau and Dmitri Starodub and Francesco Stellato and Stephan Stern and Lothar Str{\"u}lder and Nicusor Timneanu and Joachim Ullrich and Wahlgren, {Weixiao Y.} and Xiaoyu Wang and Georg Weidenspointner and Cornelia Wunderer and Petra Fromme and Chapman, {Henry N.} and John Spence and Richard Neutze",

note = "Funding Information: Experiments were carried out at the LCLS, a national user facility operated by Stanford University on behalf of the US Department of Energy, Office of Basic Energy Sciences. We acknowledge financial support from the Swedish Research Council (Vetenskapsr{\aa}det), the Swedish Foundation for International Cooperation in Research and Higher Education, Stiftelsen Olle Engkvist Byggm{\"a}stare, the Max Planck Society for funding the development and operation of the CAMP instrument, the US National Science Foundation grant MCB 0919195, the US Department of Energy Office of Basic Energy Sciences through the Photon Ultrafast Laser Science and Engineering Center Institute at the SLAC National Accelerator Laboratory and the Energy Frontier Research Center for Bio-Inspired Solar Fuel Production (award DE-SC0001016), the Hamburg Ministry of Science and Research and Joachim Herz Stiftung as part of the Hamburg Initiative for Excellence in Research and the Hamburg School for Structure and Dynamics in Infection, US National Science Foundation (awards 0417142 and MCB-1021557), US National Institutes of Health (awards 1R01GM095583-01 and 1U54GM094625-01), the Deutsche Forschungsgemeinschaft Cluster of Excellence at the Munich Center for Advanced Photonics, Center for Biophotonics Science and Technology at the University of California (cooperative agreement PHY 0120999).",

year = "2012",

month = mar,

doi = "10.1038/nmeth.1867",

language = "English (US)",

volume = "9",

pages = "263--265",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "3",

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TY - JOUR

T1 - Lipidic phase membrane protein serial femtosecond crystallography

AU - Johansson, Linda C.

AU - Arnlund, David

AU - White, Thomas A.

AU - Katona, Gergely

AU - Deponte, Daniel P.

AU - Weierstall, Uwe

AU - Doak, R. Bruce

AU - Shoeman, Robert L.

AU - Lomb, Lukas

AU - Malmerberg, Erik

AU - Davidsson, Jan

AU - Nass, Karol

AU - Liang, Mengning

AU - Andreasson, Jakob

AU - Aquila, Andrew

AU - Bajt, Sasa

AU - Barthelmess, Miriam

AU - Barty, Anton

AU - Bogan, Michael J.

AU - Bostedt, Christoph

AU - Bozek, John D.

AU - Caleman, Carl

AU - Coffee, Ryan

AU - Coppola, Nicola

AU - Ekeberg, Tomas

AU - Epp, Sascha W.

AU - Erk, Benjamin

AU - Fleckenstein, Holger

AU - Foucar, Lutz

AU - Graafsma, Heinz

AU - Gumprecht, Lars

AU - Hajdu, Janos

AU - Hampton, Christina Y.

AU - Hartmann, Robert

AU - Hartmann, Andreas

AU - Hauser, Gülnter

AU - Hirsemann, Helmut

AU - Holl, Peter

AU - Hunter, Mark S.

AU - Kassemeyer, Stephan

AU - Kimmel, Nils

AU - Kirian, Richard

AU - Maia, Filipe R N C

AU - Marchesini, Stefano

AU - Martin, Andrew V.

AU - Reich, Christian

AU - Rolles, Daniel

AU - Rudek, Benedikt

AU - Rudenko, Artem

AU - Schlichting, Ilme

AU - Schulz, Joachim

AU - Seibert, M. Marvin

AU - Sierra, Raymond G.

AU - Soltau, Heike

AU - Starodub, Dmitri

AU - Stellato, Francesco

AU - Stern, Stephan

AU - Strülder, Lothar

AU - Timneanu, Nicusor

AU - Ullrich, Joachim

AU - Wahlgren, Weixiao Y.

AU - Wang, Xiaoyu

AU - Weidenspointner, Georg

AU - Wunderer, Cornelia

AU - Fromme, Petra

AU - Chapman, Henry N.

AU - Spence, John

AU - Neutze, Richard

N1 - Funding Information: Experiments were carried out at the LCLS, a national user facility operated by Stanford University on behalf of the US Department of Energy, Office of Basic Energy Sciences. We acknowledge financial support from the Swedish Research Council (Vetenskapsrådet), the Swedish Foundation for International Cooperation in Research and Higher Education, Stiftelsen Olle Engkvist Byggmästare, the Max Planck Society for funding the development and operation of the CAMP instrument, the US National Science Foundation grant MCB 0919195, the US Department of Energy Office of Basic Energy Sciences through the Photon Ultrafast Laser Science and Engineering Center Institute at the SLAC National Accelerator Laboratory and the Energy Frontier Research Center for Bio-Inspired Solar Fuel Production (award DE-SC0001016), the Hamburg Ministry of Science and Research and Joachim Herz Stiftung as part of the Hamburg Initiative for Excellence in Research and the Hamburg School for Structure and Dynamics in Infection, US National Science Foundation (awards 0417142 and MCB-1021557), US National Institutes of Health (awards 1R01GM095583-01 and 1U54GM094625-01), the Deutsche Forschungsgemeinschaft Cluster of Excellence at the Munich Center for Advanced Photonics, Center for Biophotonics Science and Technology at the University of California (cooperative agreement PHY 0120999).

PY - 2012/3

Y1 - 2012/3

N2 - X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

AB - X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

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U2 - 10.1038/nmeth.1867

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VL - 9

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JO - Nature Methods

JF - Nature Methods

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ER -

Lipidic phase membrane protein serial femtosecond crystallography

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