Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Uwe Weierstall, Daniel James, Chong Wang, Thomas A. White, Dingjie Wang, Wei Liu, John Spence, R. Bruce Doak, Garrett Nelson, Petra Fromme, Raimund Fromme, Ingo Grotjohann, Christopher Kupitz, Nadia Zatsepin, Haiguang Liu, Shibom Basu, Daniel Wacker, Gye Won Han, Vsevolod Katritch, Sébastien BoutetMarc Messerschmidt, Garth J. Williams, Jason E. Koglin, M. Marvin Seibert, Markus Klinker, Cornelius Gati, Robert L. Shoeman, Anton Barty, Henry N. Chapman, Richard Kirian, Kenneth R. Beyerlein, Raymond C. Stevens, Dianfan Li, Syed T A Shah, Nicole Howe, Martin Caffrey, Vadim Cherezov

Research output: Contribution to journalArticlepeer-review

445 Scopus citations

Abstract

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Original languageEnglish (US)
Article number3309
JournalNature communications
Volume5
DOIs
StatePublished - Feb 14 2014

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General Physics and Astronomy

Fingerprint

Dive into the research topics of 'Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography'. Together they form a unique fingerprint.
  • Human Smoothened Receptor structure in complex with cyclopamine

    Weierstall, U. (Contributor), James, D. (Contributor), Wang, C. (Contributor), White, T. A. (Contributor), Wang, D. (Contributor), Liu, W. (Contributor), Spence, J. C. H. (Contributor), Bruce Doak, D. R. (Contributor), Nelson, G. (Contributor), Fromme, P. (Contributor), Fromme, R. (Contributor), Grotjohann, I. (Contributor), Kupitz, C. (Contributor), Zatsepin, N. A. (Contributor), Liu, H. (Contributor), Basu, S. (Contributor), Wacker, D. (Contributor), Won Han, G. (Contributor), Katritch, V. (Contributor), Boutet, S. (Contributor), Messerschmidt, M. (Contributor), Williams, G. J. (Contributor), Koglin, J. E. (Contributor), Marvin Seibert, S. M. (Contributor), Klinker, M. (Contributor), Gati, C. (Contributor), Shoeman, R. L. (Contributor), Barty, A. (Contributor), Chapman, H. N. (Contributor), Kirian, R. (Contributor), Beyerlein, K. R. (Contributor), Stevens, R. C. (Contributor), Li, D. (Contributor), Shah, S. T. A. (Contributor), Howe, N. (Contributor), Caffrey, M. (Contributor) & Cherezov, V. (Contributor), Protein Data Bank (PDB), Mar 5 2014

    Dataset

Cite this