Abstract
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Original language | English (US) |
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Article number | 3309 |
Journal | Nature communications |
Volume | 5 |
DOIs | |
State | Published - Feb 14 2014 |
ASJC Scopus subject areas
- General Chemistry
- General Biochemistry, Genetics and Molecular Biology
- General Physics and Astronomy
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Human Smoothened Receptor structure in complex with cyclopamine
Weierstall, U. (Contributor), James, D. (Contributor), Wang, C. (Contributor), White, T. A. (Contributor), Wang, D. (Contributor), Liu, W. (Contributor), Spence, J. C. H. (Contributor), Bruce Doak, D. R. (Contributor), Nelson, G. (Contributor), Fromme, P. (Contributor), Fromme, R. (Contributor), Grotjohann, I. (Contributor), Kupitz, C. (Contributor), Zatsepin, N. A. (Contributor), Liu, H. (Contributor), Basu, S. (Contributor), Wacker, D. (Contributor), Won Han, G. (Contributor), Katritch, V. (Contributor), Boutet, S. (Contributor), Messerschmidt, M. (Contributor), Williams, G. J. (Contributor), Koglin, J. E. (Contributor), Marvin Seibert, S. M. (Contributor), Klinker, M. (Contributor), Gati, C. (Contributor), Shoeman, R. L. (Contributor), Barty, A. (Contributor), Chapman, H. N. (Contributor), Kirian, R. (Contributor), Beyerlein, K. R. (Contributor), Stevens, R. C. (Contributor), Li, D. (Contributor), Shah, S. T. A. (Contributor), Howe, N. (Contributor), Caffrey, M. (Contributor) & Cherezov, V. (Contributor), Protein Data Bank (PDB), Mar 5 2014
DOI: 10.2210/pdb4O9R, https://www.wwpdb.org/pdb?id=pdb_00004o9r
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