Abstract
PPL-catalyzed synthesis of the precursor dipeptides of RGD as a cellular adhesion factor, Benzyl-Arg-Gly-NH2 and CBZ-Gly-Asp-NH2, was conducted in water-organic cosolvents systems. Five water-miscible organic solvents, which have some advantage over the water-immiscible organic solvent systems or the anhydrous organic solvent systems used often in protease-catalyzed synthesis of a peptide bond, were tested. The reaction condition of PPL-catalyzed synthesis of the dipeptides was optimized by examining the main factors affecting the product yield. The optimal reaction condition for the synthesis of Benzyl-Arg-Gly-NH2 was set up as pH 8.0, 15°C in 40% MeOH for 10 h with the maximum yield of 73.6%. The optimum condition for the synthesis of CBZ-Gly-Asp-NH2 was pH 7.0, 15°C in 50% MeOH for 10h with the maximum yield of 67.0%.
Original language | English (US) |
---|---|
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Preparative Biochemistry and Biotechnology |
Volume | 33 |
Issue number | 1 |
DOIs | |
State | Published - 2003 |
Externally published | Yes |
Keywords
- Dipeptide synthesis
- Lipase
- Organic solvents
- RGD
ASJC Scopus subject areas
- Biotechnology
- Biochemistry