Linkage map of the honey bee, Apis mellifera, based on RAPD markers

G. J. Hunt, R. E. Page

Research output: Contribution to journalArticle

168 Scopus citations

Abstract

A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female beer. The X locus for sex determination and genes for black body color anti malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be ~3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

Original languageEnglish (US)
Pages (from-to)1371-1382
Number of pages12
JournalGenetics
Volume139
Issue number3
StatePublished - Mar 14 1995

ASJC Scopus subject areas

  • Genetics

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    Hunt, G. J., & Page, R. E. (1995). Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics, 139(3), 1371-1382.