Linkage map of the honey bee, Apis mellifera, based on RAPD markers

G. J. Hunt, Robert Page

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female beer. The X locus for sex determination and genes for black body color anti malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be ~3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

Original languageEnglish (US)
Pages (from-to)1371-1382
Number of pages12
JournalGenetics
Volume139
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Honey
Bees
Genetic Markers
Genome Size
Malate Dehydrogenase
Haploidy
Genetic Recombination
Genes
Organism Cloning
Nucleotides
Color
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Hunt, G. J., & Page, R. (1995). Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics, 139(3), 1371-1382.

Linkage map of the honey bee, Apis mellifera, based on RAPD markers. / Hunt, G. J.; Page, Robert.

In: Genetics, Vol. 139, No. 3, 1995, p. 1371-1382.

Research output: Contribution to journalArticle

Hunt, GJ & Page, R 1995, 'Linkage map of the honey bee, Apis mellifera, based on RAPD markers', Genetics, vol. 139, no. 3, pp. 1371-1382.
Hunt, G. J. ; Page, Robert. / Linkage map of the honey bee, Apis mellifera, based on RAPD markers. In: Genetics. 1995 ; Vol. 139, No. 3. pp. 1371-1382.
@article{fd3054b98df748ecbd44625e0b7093c1,
title = "Linkage map of the honey bee, Apis mellifera, based on RAPD markers",
abstract = "A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female beer. The X locus for sex determination and genes for black body color anti malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be ~3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.",
author = "Hunt, {G. J.} and Robert Page",
year = "1995",
language = "English (US)",
volume = "139",
pages = "1371--1382",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "3",

}

TY - JOUR

T1 - Linkage map of the honey bee, Apis mellifera, based on RAPD markers

AU - Hunt, G. J.

AU - Page, Robert

PY - 1995

Y1 - 1995

N2 - A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female beer. The X locus for sex determination and genes for black body color anti malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be ~3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

AB - A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female beer. The X locus for sex determination and genes for black body color anti malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be ~3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

UR - http://www.scopus.com/inward/record.url?scp=0028929190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028929190&partnerID=8YFLogxK

M3 - Article

C2 - 7768445

AN - SCOPUS:0028929190

VL - 139

SP - 1371

EP - 1382

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 3

ER -