Abstract
By measuring O2 and CO2 exchange in mutants of the green alga Chlamydomonas reinhardtii in which genes encoding the reaction center of photosystem I (psaA or psaB) have been deleted, we found that a photosystem II-dependent electron flow using O2 as the final acceptor can be sustained in the light. However, in contrast with recent reports using other Chlamydomonas mutants (B4 and F8), we show here that CO2 fixation does not occur in the absence of photosystem I. By deleting the psaA gene in both B4 and F8 strains, we conclude that the ability of these mutants to fix CO2 in the light is due to the presence of residual amounts of photosystem I.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-68 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 416 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 13 1997 |
| Externally published | Yes |
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Keywords
- Chlamydomonas
- CO exchange
- Gene deletion
- O exchange
- Photosystem I
- Photosystem II
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I. / Cournac, Laurent; Redding, Kevin; Bennoun, Pierre; Peltier, Gilles.
In: FEBS Letters, Vol. 416, No. 1, 13.10.1997, p. 65-68.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I
AU - Cournac, Laurent
AU - Redding, Kevin
AU - Bennoun, Pierre
AU - Peltier, Gilles
PY - 1997/10/13
Y1 - 1997/10/13
N2 - By measuring O2 and CO2 exchange in mutants of the green alga Chlamydomonas reinhardtii in which genes encoding the reaction center of photosystem I (psaA or psaB) have been deleted, we found that a photosystem II-dependent electron flow using O2 as the final acceptor can be sustained in the light. However, in contrast with recent reports using other Chlamydomonas mutants (B4 and F8), we show here that CO2 fixation does not occur in the absence of photosystem I. By deleting the psaA gene in both B4 and F8 strains, we conclude that the ability of these mutants to fix CO2 in the light is due to the presence of residual amounts of photosystem I.
AB - By measuring O2 and CO2 exchange in mutants of the green alga Chlamydomonas reinhardtii in which genes encoding the reaction center of photosystem I (psaA or psaB) have been deleted, we found that a photosystem II-dependent electron flow using O2 as the final acceptor can be sustained in the light. However, in contrast with recent reports using other Chlamydomonas mutants (B4 and F8), we show here that CO2 fixation does not occur in the absence of photosystem I. By deleting the psaA gene in both B4 and F8 strains, we conclude that the ability of these mutants to fix CO2 in the light is due to the presence of residual amounts of photosystem I.
KW - Chlamydomonas
KW - CO exchange
KW - Gene deletion
KW - O exchange
KW - Photosystem I
KW - Photosystem II
UR - http://www.scopus.com/inward/record.url?scp=0030733504&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030733504&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(97)01170-8
DO - 10.1016/S0014-5793(97)01170-8
M3 - Article
VL - 416
SP - 65
EP - 68
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -