Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I

Laurent Cournac; Kevin Redding; Pierre Bennoun; Gilles Peltier

doi:10.1016/S0014-5793(97)01170-8

Limited photosynthetic electron flow but no CO₂ fixation in Chlamydomonas mutants lacking photosystem I

Laurent Cournac, Kevin Redding, Pierre Bennoun, Gilles Peltier

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

By measuring O₂ and CO₂ exchange in mutants of the green alga Chlamydomonas reinhardtii in which genes encoding the reaction center of photosystem I (psaA or psaB) have been deleted, we found that a photosystem II-dependent electron flow using O₂ as the final acceptor can be sustained in the light. However, in contrast with recent reports using other Chlamydomonas mutants (B4 and F8), we show here that CO₂ fixation does not occur in the absence of photosystem I. By deleting the psaA gene in both B4 and F8 strains, we conclude that the ability of these mutants to fix CO₂ in the light is due to the presence of residual amounts of photosystem I.

Original language	English (US)
Pages (from-to)	65-68
Number of pages	4
Journal	FEBS Letters
Volume	416
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)01170-8
State	Published - Oct 13 1997
Externally published	Yes

Keywords

CO exchange
Chlamydomonas
Gene deletion
O exchange
Photosystem I
Photosystem II

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I",

abstract = "By measuring O2 and CO2 exchange in mutants of the green alga Chlamydomonas reinhardtii in which genes encoding the reaction center of photosystem I (psaA or psaB) have been deleted, we found that a photosystem II-dependent electron flow using O2 as the final acceptor can be sustained in the light. However, in contrast with recent reports using other Chlamydomonas mutants (B4 and F8), we show here that CO2 fixation does not occur in the absence of photosystem I. By deleting the psaA gene in both B4 and F8 strains, we conclude that the ability of these mutants to fix CO2 in the light is due to the presence of residual amounts of photosystem I.",

keywords = "CO exchange, Chlamydomonas, Gene deletion, O exchange, Photosystem I, Photosystem II",

author = "Laurent Cournac and Kevin Redding and Pierre Bennoun and Gilles Peltier",

note = "Funding Information: Drs. E. Greenbaum and L. Mets are gratefully acknowledged for providing F8 and B4 strains, respectively, as well as stimulating discussions in concert with T. Owens and J. Lee. K.R. gratefully acknowledges the support of Jean-David Rochaix, work in whose lab was funded by the Swiss National Fund, and the support of an NSF (USA) Plant Biology Fellowship. We thank Dr. B. Dimon for help in mass spectrometric experiments and Drs. B. Genty and J. Lavergne for helpful discussions.",

year = "1997",

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doi = "10.1016/S0014-5793(97)01170-8",

language = "English (US)",

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pages = "65--68",

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AU - Cournac, Laurent

AU - Redding, Kevin

AU - Bennoun, Pierre

AU - Peltier, Gilles

N1 - Funding Information: Drs. E. Greenbaum and L. Mets are gratefully acknowledged for providing F8 and B4 strains, respectively, as well as stimulating discussions in concert with T. Owens and J. Lee. K.R. gratefully acknowledges the support of Jean-David Rochaix, work in whose lab was funded by the Swiss National Fund, and the support of an NSF (USA) Plant Biology Fellowship. We thank Dr. B. Dimon for help in mass spectrometric experiments and Drs. B. Genty and J. Lavergne for helpful discussions.

PY - 1997/10/13

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N2 - By measuring O2 and CO2 exchange in mutants of the green alga Chlamydomonas reinhardtii in which genes encoding the reaction center of photosystem I (psaA or psaB) have been deleted, we found that a photosystem II-dependent electron flow using O2 as the final acceptor can be sustained in the light. However, in contrast with recent reports using other Chlamydomonas mutants (B4 and F8), we show here that CO2 fixation does not occur in the absence of photosystem I. By deleting the psaA gene in both B4 and F8 strains, we conclude that the ability of these mutants to fix CO2 in the light is due to the presence of residual amounts of photosystem I.

AB - By measuring O2 and CO2 exchange in mutants of the green alga Chlamydomonas reinhardtii in which genes encoding the reaction center of photosystem I (psaA or psaB) have been deleted, we found that a photosystem II-dependent electron flow using O2 as the final acceptor can be sustained in the light. However, in contrast with recent reports using other Chlamydomonas mutants (B4 and F8), we show here that CO2 fixation does not occur in the absence of photosystem I. By deleting the psaA gene in both B4 and F8 strains, we conclude that the ability of these mutants to fix CO2 in the light is due to the presence of residual amounts of photosystem I.

KW - CO exchange

KW - Chlamydomonas

KW - Gene deletion

KW - O exchange

KW - Photosystem I

KW - Photosystem II

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