Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals

Weihua Wu, Tanmoy Dutta, Arul Varman, Aymerick Eudes, Bianca Manalansan, Dominique Loqué, Seema Singh

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Naturally, many aerobic organisms degrade lignin-derived aromatics through conserved intermediates including protocatechuate and catechol. Employing this microbial approach offers a potential solution for valorizing lignin into valuable chemicals for a potential lignocellulosic biorefinery and enabling bioeconomy. In this study, two hybrid biochemical routes combining lignin chemical depolymerization, plant metabolic engineering, and synthetic pathway reconstruction were demonstrated for valorizing lignin into value-added products. In the biochemical route 1, alkali lignin was chemically depolymerized into vanillin and syringate as major products, which were further bio-converted into cis, cis-muconic acid (ccMA) and pyrogallol, respectively, using engineered Escherichia coli strains. In the second biochemical route, the shikimate pathway of Tobacco plant was engineered to accumulate protocatechuate (PCA) as a soluble intermediate compound. The PCA extracted from the engineered Tobacco was further converted into ccMA using the engineered E. coli strain. This study reports a direct process for converting lignin into ccMA and pyrogallol as value-added chemicals, and more importantly demonstrates benign methods for valorization of polymeric lignin that is inherently heterogeneous and recalcitrant. Our approach also validates the promising combination of plant engineering with microbial chassis development for the production of value added and speciality chemicals.

Original languageEnglish (US)
Article number8420
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

Fingerprint

value added
lignin
pyrogallol
acids
tobacco
shikimate pathway
Escherichia coli
biorefining
value-added products
metabolic engineering
catechol
depolymerization
vanillin
alkalis
aromatic compounds
engineering
organisms

ASJC Scopus subject areas

  • General

Cite this

Lignin Valorization : Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals. / Wu, Weihua; Dutta, Tanmoy; Varman, Arul; Eudes, Aymerick; Manalansan, Bianca; Loqué, Dominique; Singh, Seema.

In: Scientific Reports, Vol. 7, No. 1, 8420, 01.12.2017.

Research output: Contribution to journalArticle

Wu, Weihua ; Dutta, Tanmoy ; Varman, Arul ; Eudes, Aymerick ; Manalansan, Bianca ; Loqué, Dominique ; Singh, Seema. / Lignin Valorization : Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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