Light-driven quinone reduction in heliobacterial membranes

Trevor S. Kashey, Dustin D. Luu, John C. Cowgill, Patricia L. Baker, Kevin Redding

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Photosynthetic reaction centers (RCs) evolved > 3 billion years ago and have diverged into Type II RCs reducing quinones and Type I RCs reducing soluble acceptors via iron–sulfur clusters. Photosystem I (PSI), the exemplar Type I RC, uses modified menaquinones as intermediate electron transfer cofactors, but it has been controversial if the Type I RC of heliobacteria (HbRC) uses its two bound menaquinones in the same way. The sequence of the quinone-binding site in PSI is not conserved in the HbRC, and the recently solved crystal structure of the HbRC does not reveal a quinone in the analogous site. We found that illumination of heliobacterial membranes resulted in reduction of menaquinone to menaquinol, suggesting that the HbRC can perform a function thought restricted to Type II RCs. Experiments on membranes and live cells are consistent with the hypothesis that the HbRC preferentially reduces soluble electron acceptors (e.g., ferredoxins) in low light, but switches to reducing lipophilic quinones in high light, when the soluble acceptor pool becomes full. Thus, the HbRC may represent a functional evolutionary intermediate between PSI and the Type II RCs.

    Original languageEnglish (US)
    Pages (from-to)1-9
    Number of pages9
    JournalPhotosynthesis Research
    DOIs
    StateAccepted/In press - Mar 12 2018

    Fingerprint

    Photosystem I Protein Complex
    Vitamin K 2
    quinones
    menaquinones
    photosystem I
    Quinones
    Membranes
    Light
    Electrons
    Photosynthetic Reaction Center Complex Proteins
    Ferredoxins
    Heliobacteriaceae
    photosynthetic reaction centers
    Lighting
    ferredoxins
    crystal structure
    Crystal structure
    Binding Sites
    Switches
    Cell Membrane

    Keywords

    • Heliobacteria
    • Quinone
    • Reaction centers
    • Type I reaction center
    • Type II reaction center

    ASJC Scopus subject areas

    • Biochemistry
    • Plant Science
    • Cell Biology

    Cite this

    Light-driven quinone reduction in heliobacterial membranes. / Kashey, Trevor S.; Luu, Dustin D.; Cowgill, John C.; Baker, Patricia L.; Redding, Kevin.

    In: Photosynthesis Research, 12.03.2018, p. 1-9.

    Research output: Contribution to journalArticle

    Kashey, Trevor S. ; Luu, Dustin D. ; Cowgill, John C. ; Baker, Patricia L. ; Redding, Kevin. / Light-driven quinone reduction in heliobacterial membranes. In: Photosynthesis Research. 2018 ; pp. 1-9.
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