Light Directed Solid Phase Synthesis on Patterned Polymers

Microarrays are commonly used in the analysis, identification, and quantification of the composition and properties of analytes. The typical density of microarray reactive sites is relatively low. This low density results in only weak fluorescent signals being generated from such microarrays during optical probing. Weak fluorescence signals necessitate the use of sensitive and expensive detection equipment for the analytical method. The present technology describes an array of chemically reactive sites comprising a plurality of substrates and a plurality of three-dimensional microstructures. The plurality of three-dimensional microstructures and substrates enables selective tailoring of reactivities. The higher concentration of reactive molecules enables the use of many less-sensitive analytical methods to probe the microarray. In the case of optical approaches, the sites will generally emit a higher intensity of light in a fluorescent array and provide greater contrast between reaction sites. Potential Applications This invention may be used in any microarray setting that requires analysis and processing of analytes. Benefits and Advantages Greater diagnostic sensitivity Enhanced reactive surface area with greater strength of functional properties Enhanced range of chemical reactivitiesDownload original PDFFor more information about the inventor(s) and their research, please see Dr. Woodbury's departmental webpageDr. Woodbury's directory webpage