Light and heavy fractions of fragment D: preparation and examination of fibrin-binding properties

V. A. Belitser, T. M. Pozdnjakova, T. P. Ugarova

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Tryptic fragment D obtained from bovine fibrinogen has been divided into one heavy and two light fractions (DH, DLI and DL2 respectively). Affinity chromatography on fibrin-agarose in media containing Ca2+ has proved most successful. DLI possesses no affinity for immobilized fibrin whereas that of DL2 is very high. DL2 interacts with fibrin about 3 times stronger than DH as shown by measurements of inhibitory effects on fibrin monomer polymerization and capacities for solubilizing fibrin permanently. DLI is fully inert.

Original languageEnglish (US)
Pages (from-to)807-814
Number of pages8
JournalThrombosis Research
Volume19
Issue number6
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

Keywords

  • affinity chromatography
  • complexes
  • fibrin
  • fragment D

ASJC Scopus subject areas

  • Hematology

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