TY - JOUR
T1 - LIGHT, a TNF-like molecule, costimulates T cell proliferation and is required for dendritic cell-mediated allogeneic T cell response
AU - Tamada, Koji
AU - Shimozaki, Koji
AU - Chapoval, Andrei I.
AU - Zhai, Yifan
AU - Su, Jeffery
AU - Chen, Su Fang
AU - Hsieh, Shie Liang
AU - Nagata, Shigekazu
AU - Ni, Jian
AU - Chen, Lieping
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000/4/15
Y1 - 2000/4/15
N2 - LIGHT is a recently identified member of the TNF superfamily and its receptors, herpesvirus entry mediator and lymphotoxin β receptor, are found in T cells and stromal cells. In this study, we demonstrate that LIGHT is selectively expressed on immature dendritic cells (DCs) generated from human PBMCs. In contrast, LIGHT is not detectable in DCs either freshly isolated from PBMCs or rendered mature in vitro by LPS treatment. Blockade of LIGHT by its soluble receptors, lymphotoxin β receptor-Ig or HVEM-Ig, inhibits the induction of DC-mediated primary allogeneic T cell response. Furthermore, engagement of LIGHT costimulates human T cell proliferation, amplifies the NF-κB signaling pathway, and preferentially induces the production of IFN- γ, but not IL-4, in the presence of an antigenic signal. Our results suggest that LIGHT is a costimulatory molecule involved in DC-mediated cellular immune responses.
AB - LIGHT is a recently identified member of the TNF superfamily and its receptors, herpesvirus entry mediator and lymphotoxin β receptor, are found in T cells and stromal cells. In this study, we demonstrate that LIGHT is selectively expressed on immature dendritic cells (DCs) generated from human PBMCs. In contrast, LIGHT is not detectable in DCs either freshly isolated from PBMCs or rendered mature in vitro by LPS treatment. Blockade of LIGHT by its soluble receptors, lymphotoxin β receptor-Ig or HVEM-Ig, inhibits the induction of DC-mediated primary allogeneic T cell response. Furthermore, engagement of LIGHT costimulates human T cell proliferation, amplifies the NF-κB signaling pathway, and preferentially induces the production of IFN- γ, but not IL-4, in the presence of an antigenic signal. Our results suggest that LIGHT is a costimulatory molecule involved in DC-mediated cellular immune responses.
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U2 - 10.4049/jimmunol.164.8.4105
DO - 10.4049/jimmunol.164.8.4105
M3 - Article
C2 - 10754304
AN - SCOPUS:0034655166
SN - 0022-1767
VL - 164
SP - 4105
EP - 4110
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -