Lifetimes of photosystem i and II proteins in the cyanobacterium Synechocystis sp. PCC 6803

Danny C I Yao, Daniel C. Brune, Willem Vermaas

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The half-life times of photosystem I and II proteins were determined using 15N-labeling and mass spectrometry. The half-life times (30-75 h for photosystem I components and <1-11 h for the large photosystem II proteins) were similar when proteins were isolated from monomeric vs. oligomeric complexes on Blue-Native gels, suggesting that the two forms of both photosystems can interchange on a timescale of <1 h or that only one form of each photosystem exists in thylakoids in vivo. The half-life times of proteins associated with either photosystem generally were unaffected by the absence of Small Cab-like proteins.

Original languageEnglish (US)
Pages (from-to)169-173
Number of pages5
JournalFEBS Letters
Volume586
Issue number2
DOIs
StatePublished - Jan 20 2012

Fingerprint

Synechocystis
Photosystem II Protein Complex
Cyanobacteria
Half-Life
Photosystem I Protein Complex
Proteins
Thylakoids
Interchanges
Labeling
Mass spectrometry
Mass Spectrometry
Gels

Keywords

  • Half-life time
  • Photosystem I
  • Photosystem II
  • Protein turnover
  • Small Cab-like protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Lifetimes of photosystem i and II proteins in the cyanobacterium Synechocystis sp. PCC 6803. / Yao, Danny C I; Brune, Daniel C.; Vermaas, Willem.

In: FEBS Letters, Vol. 586, No. 2, 20.01.2012, p. 169-173.

Research output: Contribution to journalArticle

Yao, Danny C I ; Brune, Daniel C. ; Vermaas, Willem. / Lifetimes of photosystem i and II proteins in the cyanobacterium Synechocystis sp. PCC 6803. In: FEBS Letters. 2012 ; Vol. 586, No. 2. pp. 169-173.
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