Abstract

We develop a mechanistic life-cycle model for endospore-forming bacteria (EFB) and test the model with experiments with a Bacillus mixed culture. The model integrates and quantifies how sporulation and germination are triggered by depletion or presence of a limiting substrate, while both substrates affect the rate of vegetative growth by a multiplicative model. Kinetic experiments show the accumulation of small spherical spores after the triggering substrate is depleted, substantially more rapid decay during sporulation than for normal decay of vegetative cells, and a higher specific substrate utilization rate for the germinating cells than that for growth of vegetative cells. Model simulations capture all of these experimental trends. According to model predictions, when a batch reactor is started, seeding with EFB spores instead of active EFB delays the onset of rapid chemical oxygen demand (COD) utilization and biomass growth, but the end points are the same. Simulated results with low aeration intensity show that germination can consume some substrate without dissolved oxygen (DO) depletion.

Original languageEnglish (US)
Pages (from-to)1012-1024
Number of pages13
JournalBiotechnology and Bioengineering
Volume104
Issue number5
DOIs
StatePublished - Dec 1 2009

Fingerprint

Endospore-Forming Bacteria
Germination
Life Cycle Stages
Life cycle
Bacteria
Spores
Kinetics
Growth
Biological Oxygen Demand Analysis
Substrates
Biomass
Bacillus
Oxygen
Chemical oxygen demand
Batch reactors
Bacilli
Dissolved oxygen
Experiments
Cells

Keywords

  • Endospore-forming bacteria
  • Germination
  • Life-cycle model
  • Sporulation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Life-cycle kinetic model for endospore-forming bacteria, including germination and sporulation. / Park, Seongjun; Rittmann, Bruce; Bae, Wookeun.

In: Biotechnology and Bioengineering, Vol. 104, No. 5, 01.12.2009, p. 1012-1024.

Research output: Contribution to journalArticle

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N2 - We develop a mechanistic life-cycle model for endospore-forming bacteria (EFB) and test the model with experiments with a Bacillus mixed culture. The model integrates and quantifies how sporulation and germination are triggered by depletion or presence of a limiting substrate, while both substrates affect the rate of vegetative growth by a multiplicative model. Kinetic experiments show the accumulation of small spherical spores after the triggering substrate is depleted, substantially more rapid decay during sporulation than for normal decay of vegetative cells, and a higher specific substrate utilization rate for the germinating cells than that for growth of vegetative cells. Model simulations capture all of these experimental trends. According to model predictions, when a batch reactor is started, seeding with EFB spores instead of active EFB delays the onset of rapid chemical oxygen demand (COD) utilization and biomass growth, but the end points are the same. Simulated results with low aeration intensity show that germination can consume some substrate without dissolved oxygen (DO) depletion.

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