TY - JOUR
T1 - Lauric Triglyceride Ameliorates High-Fat-Diet-Induced Obesity in Rats by Reducing Lipogenesis and Increasing Lipolysis and β-Oxidation
AU - Xia, Jiaheng
AU - Yu, Ping
AU - Zeng, Zheling
AU - Ma, Maomao
AU - Zhang, Guohua
AU - Wan, Dongman
AU - Gong, Deming
AU - Deng, Shuguang
AU - Wang, Jun
N1 - Funding Information:
This work was supported by the International Science and Technology Cooperation Program of China (Project 2011DFA32770), the Research Program of the State Key Laboratory of Food Science and Technology, Nanchang University (Projects SKLF-ZZB-201517, SKLF-ZZA-201610, SKLF-KF-201812, and SKLF-ZZB-201916), the Science and Technology Program of Jiangxi Province (Project 20143ACG70015), and the National Natural Science Foundation of China (Projects 31701651 and 32060516).
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/8/18
Y1 - 2021/8/18
N2 - Medium-chain triglycerides (MCTs) are found in limited foods. In these medium-chain oil resources, the abundance of lauric acid (LA) is the highest among medium-chain fatty acids (MCFAs), and its effects on lipid metabolism in obese rats have not been well-studied. This study aimed to determine the anti-obesity effects and mechanisms of lauric triglyceride (LT) in Sprague Dawley (SD) rats. LA and glycerin were used to synthesize LT, then LT was used to treat obese rats for 12 weeks. The results showed that LT significantly reduced the body weight, body mass index, and Lee's index in obese rats. The mRNA expression levels of the anorexic neuropeptide POMC in the hypothalamus between the LT group and the other groups were not different, while the gene expression levels of the orexigenic neuropeptides NPY and AGRP decreased significantly in the LT group. Except serum cholesterol, LT improved the serum triglyceride metabolism in the obese rats and reduced adipocyte and hepatic lipid deposition. Moreover, LT inhibited the expression of lipogenesis-related genes and proteins (SREBP-1c, ACC1, and FASN) and increased the expression of lipolysis (ATGL, HSL, and LPL) and β-oxidation (PPARα, CPT-1a, and PCG-1α) related genes and proteins in the white fat and liver. Furthermore, LT increased the mRNA expression of mitochondrial-biosynthesis-related genes (SIRT1, NRF1, and TFAM) in the liver. The results indicated that LT ameliorates diet-induced obesity in rats.
AB - Medium-chain triglycerides (MCTs) are found in limited foods. In these medium-chain oil resources, the abundance of lauric acid (LA) is the highest among medium-chain fatty acids (MCFAs), and its effects on lipid metabolism in obese rats have not been well-studied. This study aimed to determine the anti-obesity effects and mechanisms of lauric triglyceride (LT) in Sprague Dawley (SD) rats. LA and glycerin were used to synthesize LT, then LT was used to treat obese rats for 12 weeks. The results showed that LT significantly reduced the body weight, body mass index, and Lee's index in obese rats. The mRNA expression levels of the anorexic neuropeptide POMC in the hypothalamus between the LT group and the other groups were not different, while the gene expression levels of the orexigenic neuropeptides NPY and AGRP decreased significantly in the LT group. Except serum cholesterol, LT improved the serum triglyceride metabolism in the obese rats and reduced adipocyte and hepatic lipid deposition. Moreover, LT inhibited the expression of lipogenesis-related genes and proteins (SREBP-1c, ACC1, and FASN) and increased the expression of lipolysis (ATGL, HSL, and LPL) and β-oxidation (PPARα, CPT-1a, and PCG-1α) related genes and proteins in the white fat and liver. Furthermore, LT increased the mRNA expression of mitochondrial-biosynthesis-related genes (SIRT1, NRF1, and TFAM) in the liver. The results indicated that LT ameliorates diet-induced obesity in rats.
KW - anti-obesity
KW - lauric triglyceride
KW - lipid metabolism
KW - lipogenesis
KW - lipolysis
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U2 - 10.1021/acs.jafc.0c07342
DO - 10.1021/acs.jafc.0c07342
M3 - Article
C2 - 33433211
AN - SCOPUS:85099835880
VL - 69
SP - 9157
EP - 9166
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 32
ER -