Laser welding of ruptured intestinal tissue using plasmonic polypeptide nanocomposite solders

Huang Chiao Huang, Candace Rae Walker, Alisha Nanda, Kaushal Rege

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Approximately 1.5 million people suffer from colorectal cancer and inflammatory bowel disease in the United States. Occurrence of leakage following standard surgical anastomosis in intestinal and colorectal surgery is common and can cause infection leading to life-threatening consequences. In this report, we demonstrate that plasmonic nanocomposites, generated from elastin-like polypeptides (ELPs) cross-linked with gold nanorods, can be used to weld ruptured intestinal tissue upon exposure to near-infrared (NIR) laser irradiation. Mechanical properties of these nanocomposites can be modulated based on the concentration of gold nanorods embedded within the ELP matrix. We employed photostable, NIR-absorbing cellularized and noncellularized GNR-ELP nanocomposites for ex vivo laser welding of ruptured porcine small intestines. Laser welding using the nanocomposites significantly enhanced the tensile strength, leakage pressure, and bursting pressure of ruptured intestinal tissue. This, in turn, provided a liquid-tight seal against leakage of luminal liquid from the intestine and resulting bacterial infection. This study demonstrates the utility of laser tissue welding using plasmonic polypeptide nanocomposites and indicates the translational potential of these materials in intestinal and colorectal repair.

Original languageEnglish (US)
Pages (from-to)2988-2998
Number of pages11
JournalACS Nano
Volume7
Issue number4
DOIs
StatePublished - Apr 23 2013

Fingerprint

Welding
Nanocomposites
laser welding
Laser beam welding
Polypeptides
polypeptides
solders
Soldering alloys
nanocomposites
elastin
Lasers
Elastin
Tissue
Peptides
Nanotubes
intestines
leakage
infectious diseases
Nanorods
Gold

Keywords

  • elastin-like polypeptide
  • gold nanorods
  • laser tissue welding
  • plasmonic biomaterials

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Medicine(all)

Cite this

Laser welding of ruptured intestinal tissue using plasmonic polypeptide nanocomposite solders. / Huang, Huang Chiao; Walker, Candace Rae; Nanda, Alisha; Rege, Kaushal.

In: ACS Nano, Vol. 7, No. 4, 23.04.2013, p. 2988-2998.

Research output: Contribution to journalArticle

Huang, Huang Chiao ; Walker, Candace Rae ; Nanda, Alisha ; Rege, Kaushal. / Laser welding of ruptured intestinal tissue using plasmonic polypeptide nanocomposite solders. In: ACS Nano. 2013 ; Vol. 7, No. 4. pp. 2988-2998.
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