Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.

Original languageEnglish (US)
Article number89
JournalBMC Biotechnology
Issue number1
StatePublished - Dec 19 2017



  • Adhesion
  • Cell culture
  • Cell patterning
  • Microwell
  • Single-cell

ASJC Scopus subject areas

  • Biotechnology

Cite this