Abstract

Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.

Original languageEnglish (US)
Article number89
JournalBMC Biotechnology
Volume17
Issue number1
DOIs
StatePublished - Dec 19 2017

Fingerprint

Single-Cell Analysis
Lasers
Bioengineering
Cell Separation
Polymers
Phenotype

Keywords

  • Adhesion
  • Cell culture
  • Cell patterning
  • Microwell
  • Single-cell

ASJC Scopus subject areas

  • Biotechnology

Cite this

Laser-fabricated cell patterning stencil for single cell analysis. / Messner, Jacob J.; Glenn, Honor L.; Meldrum, Deirdre.

In: BMC Biotechnology, Vol. 17, No. 1, 89, 19.12.2017.

Research output: Contribution to journalArticle

Messner, Jacob J. ; Glenn, Honor L. ; Meldrum, Deirdre. / Laser-fabricated cell patterning stencil for single cell analysis. In: BMC Biotechnology. 2017 ; Vol. 17, No. 1.
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