Abstract
Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.
Original language | English (US) |
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Article number | 89 |
Journal | BMC Biotechnology |
Volume | 17 |
Issue number | 1 |
DOIs | |
State | Published - Dec 19 2017 |
Keywords
- Adhesion
- Cell culture
- Cell patterning
- Microwell
- Single-cell
ASJC Scopus subject areas
- Biotechnology