Laser-captured microglia in the Alzheimer's and Parkinson's brain reveal unique regional expression profiles and suggest a potential role for hepatitis B in the Alzheimer's brain

Diego Mastroeni, Jennifer Nolz, Shobana Sekar, Elaine Delvaux, Geidy Serrano, Lori Cuyugan, Winnie S. Liang, Thomas G. Beach, Joseph Rogers, Paul Coleman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Expression array data from dozens of laboratories, including our own, show significant changes in expression of many genes in Alzheimer's disease (AD) patients compared with normal controls. These data typically rely on brain homogenates, and information about transcripts specific to microglia and other central nervous system (CNS) cell types, which far outnumber microglia-specific transcripts, is lost. We therefore used single-cell laser capture methods to assess the full range of microglia-specific expression changes that occur in different brain regions (substantia nigra and hippocampus CA1) and disease states (AD, Parkinson's disease, and normal controls). Two novel pathways, neuronal repair and viral processing were identified. Based on KEGG analysis (Kyoto Encyclopedia of Genes and Genomes, a collection of biological pathways), one of the most significant viruses was hepatitis B virus (HBV) (false discovery rate < 0.00000001). Immunohistochemical analysis using HBV-core antibody in HBV-positive control, amnestic mild cognitive impairment, and HBV-positive AD cases show increased HBV immunoreactivity as disease pathology increases. These results are the first, to our knowledge, to show regional differences in human microglia. In addition, these data reveal new functions for microglia and suggest a novel risk factor for AD.

Original languageEnglish (US)
Pages (from-to)12-21
Number of pages10
JournalNeurobiology of Aging
Volume63
DOIs
StatePublished - Mar 1 2018

Fingerprint

Microglia
Hepatitis B
Lasers
Hepatitis B virus
Alzheimer Disease
Brain
Encyclopedias
Hepatitis B Antibodies
Substantia Nigra
Parkinson Disease
Hippocampus
Central Nervous System
Genome
Pathology
Viruses
Gene Expression
Genes

Keywords

  • Alzheimer's disease
  • Laser capture
  • Microglia
  • Parkinson's disease
  • RNA sequencing
  • Synapse
  • Virus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

Cite this

Laser-captured microglia in the Alzheimer's and Parkinson's brain reveal unique regional expression profiles and suggest a potential role for hepatitis B in the Alzheimer's brain. / Mastroeni, Diego; Nolz, Jennifer; Sekar, Shobana; Delvaux, Elaine; Serrano, Geidy; Cuyugan, Lori; Liang, Winnie S.; Beach, Thomas G.; Rogers, Joseph; Coleman, Paul.

In: Neurobiology of Aging, Vol. 63, 01.03.2018, p. 12-21.

Research output: Contribution to journalArticle

Mastroeni, Diego ; Nolz, Jennifer ; Sekar, Shobana ; Delvaux, Elaine ; Serrano, Geidy ; Cuyugan, Lori ; Liang, Winnie S. ; Beach, Thomas G. ; Rogers, Joseph ; Coleman, Paul. / Laser-captured microglia in the Alzheimer's and Parkinson's brain reveal unique regional expression profiles and suggest a potential role for hepatitis B in the Alzheimer's brain. In: Neurobiology of Aging. 2018 ; Vol. 63. pp. 12-21.
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