Large deletion mutants of Escherichia coli tRNA1Tyr

George M. McCorkle, Sidney Altman

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Two suppressor-negative derivatives (9311 and 9313) of bacteriophage φ80su3+ have been shown to contain deletions of 110 base-pairs starting in the 3′-terminal part of the tRNA1Tyr gene and extending downstream. These mutations delete a 3′-proximal RNA processing site, thereby leading to the accumulation of an abnormal, unstable gene transcript. One of the suppressor-negative derivatives (9313) contains a second deletion of 178 base-pairs further downstream from the first one. The ability of 9313 to regain the parental su3+ phenotype with a frequency of 1% may be related to the presence of the second deletion. The deletions, which resulted originally from an ICR364-OH (an aza-quinacrine compound)-induced mutagenic event, have exactly repeated sequences of nine and six base-pairs, respectively, at their termini.

Original languageEnglish (US)
Pages (from-to)83-103
Number of pages21
JournalJournal of molecular biology
Volume155
Issue number2
DOIs
StatePublished - Feb 25 1982
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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