Lactoperoxidase-catalyzed iodination of chloroplast membranes. I. Analysis of surface-localized proteins

C. J. Arntzen, P. A. Armond, C. S. Zettinger, C. Vernotte, J. M. Briantais

Research output: Contribution to journalArticle

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Abstract

Lactoperoxidase-catalyzed iodination of chloroplast membranes has been employed to characterize the vectorial distribution of lamellar proteins. The enzymatic reaction is highly specific for only the outermost membrane components (Phillips, D. R. and Morrison, M. (1971) Biochemistry 10, 1766-1771); we have determined the distribution of 125I label and changes in photochemical activities after iodination in an effort to identify these components. Three major conclusions are evident: 1. 1. The coupling factor for photophosphorylation is highly exposed and is selectively and rapidly inhibited by the iodination reaction. 2. 2. A loss of Photosystem I activity (NADP reduction) resulted from iodination. Partial reactions indicated the effect was on electron-transport components on the reducing side of Photosystem I. There was also a limited inhibition of methyl viologen reduction. 3. 3. Iodination of intact membranes caused a reduction in rates of Photosystem II-dependent Hill reaction activity. This inhibition could not be explained solely on the basis of iodination effects on electron-transport components involved in the oxidation of water. The implications of these data with respect to previous chloroplast-membrane models are discussed.

Original languageEnglish (US)
Pages (from-to)329-339
Number of pages11
JournalBBA - Bioenergetics
Volume347
Issue number3
DOIs
StatePublished - Jun 28 1974
Externally publishedYes

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Lactoperoxidase
Halogenation
Chloroplasts
Membrane Proteins
Membranes
Photosystem I Protein Complex
Proteins
Electron Transport
Biochemistry
Paraquat
Photosystem II Protein Complex
NADP
Photophosphorylation
Labels
Oxidation
Water

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

Lactoperoxidase-catalyzed iodination of chloroplast membranes. I. Analysis of surface-localized proteins. / Arntzen, C. J.; Armond, P. A.; Zettinger, C. S.; Vernotte, C.; Briantais, J. M.

In: BBA - Bioenergetics, Vol. 347, No. 3, 28.06.1974, p. 329-339.

Research output: Contribution to journalArticle

Arntzen, C. J. ; Armond, P. A. ; Zettinger, C. S. ; Vernotte, C. ; Briantais, J. M. / Lactoperoxidase-catalyzed iodination of chloroplast membranes. I. Analysis of surface-localized proteins. In: BBA - Bioenergetics. 1974 ; Vol. 347, No. 3. pp. 329-339.
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