Lack of norovirus replication and histo-blood group antigen expression in 3-dimensional intestinal epithelial cells

Melissa M. Herbst-Kralovetz, Andrea L. Radtke, Margarita K. Lay, Brooke E. Hjelm, Alice N. Bolick, Shameema S. Sarker, Robert L. Atmar, David H. Kingsley, Charles J. Arntzen, Mary K. Estes, Cheryl Nickerson

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Abstract

Noroviruses (NoVs) are a leading cause of gastroenteritis worldwide. An in vitro model for NoV replication remains elusive, making study of the virus difficult. A previous study, which used a 3-dimensional (3-D) intestinal model derived from INT-407 cells reported NoV replication and extensive cytopathic effects (CPE). Using the same 3-D model, but with highly purified Norwalk virus (NV), we attempted to replicate this study. Our results showed no evidence of NV replication by real-time PCR of viral RNA or by immunocytochemical detection of viral structural and nonstructural proteins. Immunocytochemical analysis of the 3-D cultures also showed no detectable presence of histo-blood group antigens that participate in NV binding and host tropism. To determine the potential cause of CPE observed in the previous study, we exposed 3-D cultures to lipopolysaccharide concentrations consistent with contaminated stool samples and observed morphologic features similar to CPE. We conclude that the 3-D INT-407 model does not support NV replication.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalEmerging Infectious Diseases
Volume19
Issue number3
DOIs
StatePublished - Mar 1 2013

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ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Herbst-Kralovetz, M. M., Radtke, A. L., Lay, M. K., Hjelm, B. E., Bolick, A. N., Sarker, S. S., Atmar, R. L., Kingsley, D. H., Arntzen, C. J., Estes, M. K., & Nickerson, C. (2013). Lack of norovirus replication and histo-blood group antigen expression in 3-dimensional intestinal epithelial cells. Emerging Infectious Diseases, 19(3), 431-438. https://doi.org/10.3201/eid1903.121029