Abstract
Nanogravimetry label-free applications to the Nucleic Acid Programmed Protein Array technology is reviewed. DNA micro-arrays constitute a major advance in functional proteomics for the in situ generation of proteins micro arrays. The use of two different nanogravimetric technologies allows to investigate the kinetics during the in situ proteins expression. The first nanogravimetric apparatus is capable to detect frequency variations and protein production kinetics from four independent transducers driven in parallel, with a miniature flow-cell to follow each step of the protein expression protocol directly on the quartz. A software tool able to drive the signal acquisition and to analyze the acquired data was developed. First results on the in situ expression of proteins are reported. The second nanogravimetric instrumentation has been developed since very recently to account for viscoelastic behavior of protein films smoothly connected to the quartz crystal in terms of the quality factor. The system can detect also the crystal oscillations variations at expressing proteins in a liquid environment. The complex nature of these experiments is briefly discussed and preliminary results reported.
| Original language | English (US) |
|---|---|
| Title of host publication | Functional Proteomics and Nanotechnology-Based Microarrays |
| Publisher | Pan Stanford Publishing Pte. Ltd. |
| Pages | 79-93 |
| Number of pages | 15 |
| ISBN (Print) | 9789814267762 |
| DOIs | |
| State | Published - Jun 1 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Label free NAPPA via nanogravimetry. / Adami, Manuela; Sallam, Sameh; Eggenhöffner, Roberto; Sartore, Marco; Hainsworth, Eugenie; LaBaer, Joshua; Nicolini, Claudio.
Functional Proteomics and Nanotechnology-Based Microarrays. Pan Stanford Publishing Pte. Ltd., 2010. p. 79-93.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Label free NAPPA via nanogravimetry
AU - Adami, Manuela
AU - Sallam, Sameh
AU - Eggenhöffner, Roberto
AU - Sartore, Marco
AU - Hainsworth, Eugenie
AU - LaBaer, Joshua
AU - Nicolini, Claudio
PY - 2010/6/1
Y1 - 2010/6/1
N2 - Nanogravimetry label-free applications to the Nucleic Acid Programmed Protein Array technology is reviewed. DNA micro-arrays constitute a major advance in functional proteomics for the in situ generation of proteins micro arrays. The use of two different nanogravimetric technologies allows to investigate the kinetics during the in situ proteins expression. The first nanogravimetric apparatus is capable to detect frequency variations and protein production kinetics from four independent transducers driven in parallel, with a miniature flow-cell to follow each step of the protein expression protocol directly on the quartz. A software tool able to drive the signal acquisition and to analyze the acquired data was developed. First results on the in situ expression of proteins are reported. The second nanogravimetric instrumentation has been developed since very recently to account for viscoelastic behavior of protein films smoothly connected to the quartz crystal in terms of the quality factor. The system can detect also the crystal oscillations variations at expressing proteins in a liquid environment. The complex nature of these experiments is briefly discussed and preliminary results reported.
AB - Nanogravimetry label-free applications to the Nucleic Acid Programmed Protein Array technology is reviewed. DNA micro-arrays constitute a major advance in functional proteomics for the in situ generation of proteins micro arrays. The use of two different nanogravimetric technologies allows to investigate the kinetics during the in situ proteins expression. The first nanogravimetric apparatus is capable to detect frequency variations and protein production kinetics from four independent transducers driven in parallel, with a miniature flow-cell to follow each step of the protein expression protocol directly on the quartz. A software tool able to drive the signal acquisition and to analyze the acquired data was developed. First results on the in situ expression of proteins are reported. The second nanogravimetric instrumentation has been developed since very recently to account for viscoelastic behavior of protein films smoothly connected to the quartz crystal in terms of the quality factor. The system can detect also the crystal oscillations variations at expressing proteins in a liquid environment. The complex nature of these experiments is briefly discussed and preliminary results reported.
UR - http://www.scopus.com/inward/record.url?scp=84881760612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881760612&partnerID=8YFLogxK
U2 - 10.4032/9789814267779
DO - 10.4032/9789814267779
M3 - Chapter
AN - SCOPUS:84881760612
SN - 9789814267762
SP - 79
EP - 93
BT - Functional Proteomics and Nanotechnology-Based Microarrays
PB - Pan Stanford Publishing Pte. Ltd.
ER -